Description

  • Product name

    Recombinant mouse PDGF AA protein
    See all PDGF AA proteins and peptides
  • Biological activity

    Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/ml.
  • Purity

    > 95 % SDS-PAGE.
    Purity Greater than 97% as determined by reducing and non-reducing SDS-PAGE, and by analytical HPLC. This product was sterile filtered.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT

Specifications

Our Abpromise guarantee covers the use of ab78590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute the lyophilized product with sterile water at a concentration of 0.1 -0.5 mg's/ml, which can be further diluted into other aqueous solutions.

General Info

  • Alternative names

    • PDGF A
    • PDGF A chain
    • PDGF subunit A
    • PDGF-1
    • PDGF1
    • PDGFA
    • PDGFA_HUMAN
    • Platelet derived growth factor alpha chain
    • Platelet derived growth factor alpha isoform 2 preproprotein
    • Platelet derived growth factor alpha polypeptide
    • Platelet-derived growth factor A chain
    • Platelet-derived growth factor alpha polypeptide
    • Platelet-derived growth factor subunit A
    see all
  • Function

    Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Domain

    The long form contains a basic insert which acts as a cell retention signal.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  •  SDS PAGE analysis of ab78590 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

  • ab78590 used in Functional Studies.

References

ab78590 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank for your enquiry and interest.





I would like to confirm the this kit contains 2 vials of Standards: recombinant human PDGF-AA





However, currently we also have we have 4 products PDGF AA protein. These products have not been tested together specifically with the kit ab100622.

ab73226: tested in functional studies, SDS-PAGE
ab9702: tested in functional studies, SDS-PAGE
ab53372: SDS-PAGE
ab78590: SDS-PAGE





I hope this helps and if I can assist further, please do not hesitate to contact me.

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