Recombinant Mouse PDK4 protein (His tag) (ab235847)

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SDS-PAGE - Recombinant Mouse PDK4 protein (His tag) (ab235847)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Mouse PDK4 protein (His tag)
      See all PDK4 proteins and peptides

    • Purity

      > 85 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      O70571

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mouse

      • Sequence

        ILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSKTGN PSHIGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFPGQ PIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPVEATVVLGKEDL TIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPIS RLYAKYFQGDLNLYSMSGYGTDAIIYLKALS

      • Predicted molecular weight

        30 kDa including tags

      • Amino acids

        138 to 368

      • Tags

        His tag N-Terminus

      • Additional sequence information

        N-terminal 6xHis-tagged.

    Specifications

    Our Abpromise guarantee covers the use of ab235847 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
      • FLJ40832
      • mitochondrial
      • Pdk4
      • PDK4_HUMAN
      • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial
      • Pyruvate dehydrogenase kinase 4
      • Pyruvate dehydrogenase kinase isoenzyme 4
      • Pyruvate dehydrogenase kinase isoform 4
      • Pyruvate dehydrogenase kinase isozyme 4
      • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
      see all

    • Function

      Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

    • Tissue specificity

      Ubiquitous; highest levels of expression in heart and skeletal muscle.

    • Sequence similarities

      Belongs to the PDK/BCKDK protein kinase family.
      Contains 1 histidine kinase domain.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Mouse PDK4 protein (His tag) (ab235847)
      SDS-PAGE - Recombinant Mouse PDK4 protein (His tag) (ab235847)

      ab235847 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab235847? Please let us know so that we can cite the reference in this datasheet.

    ab235847 has not yet been referenced specifically in any publications.

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