Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant mouse Peroxiredoxin 1/PAG protein (Active)
See all Peroxiredoxin 1/PAG proteins and peptides
Specific activity is >2,500 pmol/min/μg. Enzymatic activity is defined as the amount of hydroperoxide that 1μg of enzyme can reduce at 25°C for minute.
Purity> 90 % SDS-PAGE.
purified by using conventional chromatography techniques.
Endotoxin level< 1.000 Eu/µg
Expression systemBaculovirus infected insect cells
Protein lengthFull length protein
Predicted molecular weight23 kDa including tags
Amino acids1 to 199
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab219223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Heme binding 23 kDa protein
- Natural killer cell-enhancing factor A
FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
modificationsPhosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219223 has not yet been referenced specifically in any publications.