Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Molecular weight

      63 kDa including tags
    • Amino acids

      1 to 326

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab125561 is 785 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity

    > 90 % Densitometry.
    ab125561 is >90% pure.
  • Form

    Liquid
  • Additional notes

    ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Kid 1
    • Kid1
    • Kinase induced by depolarization
    • ONCOGENE PIM3
    • PIM 3
    • Pim 3 oncogene
    • Pim-3 proto-oncogene, serine/threonine kinase
    • PIM3
    • Pim3 oncogene
    • PIM3_HUMAN
    • Protein kinase Kid 1
    • Protein kinase Kid1
    • Serine/threonine kinase Pim 3
    • Serine/threonine kinase Pim3
    • Serine/threonine protein kinase Pim 3
    • Serine/threonine protein kinase Pim3
    • Serine/threonine-protein kinase Pim-3
    see all
  • Function

    Able to phosphorylate CDKN1B in vitro. Involved in cell cycle progression and suppression of apoptosis. Implicated in proliferation of human hepatoma cell lines.
  • Tissue specificity

    Widely expressed. No expression in colon, thymus, and small intestine. Expressed in human hepatoma cell lines but not in normal liver tissues. Expressed in colon cancer samples, particularly at the less advanced stages of cancer development.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

Images

  • The specific activity of ab125561 was determined to be 785 nmol/min/mg.
  • SDS PAGE analysis of ab125561 (PIM3 protein).

References

ab125561 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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