Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGSMPKPHSEAGTAFIQTQQLHAAMADTFL EHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFS HGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGS GTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVD DGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQ DLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGV RRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICA TQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRM QHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGA IIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDA VLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRV VPVP
    • Molecular weight
      60 kDa including tags
    • Amino acids
      1 to 531
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab223006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity: > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.

  • Applications

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 85 % SDS-PAGE.
    ab223006 was urified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.5
    Constituents: 0.32% Tris HCl, 1.16% Sodium chloride, 0.02% DTT, 30% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CTHBP
    • Cytosolic thyroid hormone binding protein
    • Cytosolic thyroid hormone-binding protein
    • KPYM_HUMAN
    • MGC3932
    • OIP 3
    • OIP-3
    • OIP3
    • OPA interacting protein 3
    • Opa-interacting protein 3
    • p58
    • PK muscle type
    • PK, muscle type
    • PK2
    • PK3
    • PKM
    • PKM2
    • pykm
    • Pyruvate kinase 2/3
    • Pyruvate kinase 3
    • Pyruvate kinase isozymes M1/M2
    • Pyruvate kinase muscle
    • Pyruvate kinase muscle isozyme
    • pyruvate kinase PKM
    • Pyruvate kinase, muscle 2
    • TCB
    • THBP1
    • Thyroid hormone binding protein 1
    • Thyroid hormone binding protein cytosolic
    • Thyroid hormone-binding protein 1
    • Tumor M2 PK
    • Tumor M2-PK
    see all
  • Function
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities
    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt

Images

  • 15% SDS-PAGE - Recombinant mouse PKM2 protein (Active) (3 µg ab223006).

References

ab223006 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223006.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up