Recombinant mouse PRMT1 protein (ab78937)

Submit a review Submit a question
SDS-PAGE - Recombinant mouse PRMT1 protein (ab78937)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant mouse PRMT1 protein
      See all PRMT1 proteins and peptides

    • Biological activity

      Specific Activity: >30nmol/min/mg.  Unit Definition: The amount of enzyme that transfer 1nmole of methyl group per minute at 37°C.

    • Purity

      > 90 % SDS-PAGE.
      Purified by using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mouse

      • Sequence

        MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

      • Amino acids

        1 to 353

      • Additional sequence information

        This protein has a His-MBP tag at the N-terminus.

    Specifications

    Our Abpromise guarantee covers the use of ab78937 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.038% Magnesium chloride, 0.0308% DTT, 0.484% Tris, 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • ANM 1
      • ANM1
      • ANM1_HUMAN
      • HCP 1
      • HCP1
      • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2
      • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2
      • Heterogeneous nuclear ribonucleoproteins methyltransferase like2
      • Histone-arginine N-methyltransferase PRMT1
      • HMT 2
      • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2
      • HMT1 hnRNP methyltransferase
      • HMT1 hnRNP methyltransferase like 2
      • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae)
      • HMT2
      • HRMT1 L2
      • HRMT1L 2
      • HRMT1L2
      • Human mRNA for suppressor for yeast mutant
      • Human mRNA for suppressor for yeast mutant complete cds
      • Interferon receptor 1 bound protein 4
      • Interferon receptor 1 bound protein4
      • Interferon receptor 1-bound protein 4
      • Interferon receptor 1bound protein 4
      • IR1 B4
      • IR1B 4
      • IR1B4
      • Mrmt 1
      • Mrmt1
      • PRMT 1
      • PRMT1
      • Protein arginine methyltransferase 1
      • Protein arginine N methyltransferase 1
      • Protein arginine N methyltransferase1
      • Protein arginine N-methyltransferase 1
      • R1B4
      • S. cerevisiae like 2
      see all

    • Function

      Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the protein arginine N-methyltransferase family.

    • Cellular localization

      Nucleus. Cytoplasm > cytosol.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant mouse PRMT1 protein (ab78937)
      SDS-PAGE - Recombinant mouse PRMT1 protein (ab78937)
      ab78937 on 15% SDS-PAGE, 3µg.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab78937? Please let us know so that we can cite the reference in this datasheet.

    ab78937 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab78937.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com