Description

  • Product name

    Recombinant Mouse Procalcitonin protein
    See all Procalcitonin proteins and peptides
  • Purity

    > 94 % Densitometry.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MKHHHHHHAS VPLRSILESS PGMATLSEEE VRLLAALVQD YMQMKARELE QEEEQEAEGS SLDSPRSKRC GNLSTCMLGT YTQDLNKFHT FPQTSIGVEA PGKKRDVAKD LETNHQSHFG N
    • Predicted molecular weight

      14 kDa including tags
    • Amino acids

      26 to 136
    • Tags

      His tag N-Terminus
    • Additional sequence information

      This is the full length protein without the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab203548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Mass Spectrometry

    SDS-PAGE

    ELISA

  • Mass spectrometry

    LC-MS/MS
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.5
    Constituents: 0.24% Tris buffer, 0.29% Sodium chloride

  • Reconstitution
    Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

General Info

  • Alternative names

    • CALC1
    • CALCA
    • calcitonin related polypeptide alpha
    • CGRP
    • CGRP1
    • CT
    • katacalcin
    • KC
    see all
  • Relevance

    Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.
  • Cellular localization

    Secreted

Images

  • 14% SDS-PAGE separation of ab203548.
    1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
    2. Reduced and heated sample, 2.5 μg / lane
    3. Non-reduced and non-heated sample, 2.5 μg / lane

References

ab203548 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203548.
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