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  1. Link

    recombinant-mouse-rankl-protein-active-ab129136.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Bioactive grade

Recombinant mouse RANKL protein (Active) (ab129136)

  • Datasheet
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Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)
  • Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)
  • SDS-PAGE - Recombinant mouse RANKL protein (Active) (ab129136)

Key features and details

  • Expression system: Escherichia coli
  • Purity: >= 95% SDS-PAGE
  • Endotoxin level: <= 1.000 Eu/µg
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Achieve higher consistency and quality standards with a premium grade bioactive protein

Product image
Recombinant mouse RANKL protein (Active) (ab270070)
  • High batch-to-batch consistency
  • Optimal bioactivity
  • Guaranteed identical to human native proteins
  • >95% purity
  • Ultra-low endotoxin levels: <0.005 Eu/µg
  • Carrier and tag free

Description

  • Product name

    Recombinant mouse RANKL protein (Active)
    See all RANKL proteins and peptides
  • Biological activity

    ab129136 activity is measured by its ability to induce osteoclast formation on mouse RAW264.7 cells using a concentration of 5.0-10.0 ng/mL (or ≥ 2.0 x 10^4 units/mg).

  • Purity

    >= 95 % SDS-PAGE.
    UV spectroscopy at 280 nm. Purified using standard chromatographical techniques.
  • Endotoxin level

    <=1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

    O35235
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MPAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID
    • Predicted molecular weight

      20 kDa
    • Amino acids

      143 to 316

Specifications

Our Abpromise guarantee covers the use of ab129136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride

    Lyophilized from a sterile (0.2 micron) filtered aqueous solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. Gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Allow several minutes for complete reconstitution.

General Info

  • Alternative names

    • CD254
    • hRANKL2
    • ODF
    • OPGL
    • OPTB2
    • Osteoclast differentiation factor
    • Osteoprotegerin ligand
    • Rank Ligand
    • RANKL
    • Receptor activator of nuclear factor kappa B ligand
    • Receptor activator of nuclear factor kappa-B ligand
    • sOdf
    • TNF related activation induced cytokine
    • TNF-related activation-induced cytokine
    • TNF11_HUMAN
    • TNFSF 11
    • Tnfsf11
    • TRANCE
    • Tumor necrosis factor (ligand) superfamily member 11
    • Tumor necrosis factor ligand superfamily member 11
    • Tumor necrosis factor ligand superfamily member 11, soluble form
    see all
  • Function

    Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • Tissue specificity

    Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • Involvement in disease

    Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • Cellular localization

    Cytoplasm; Secreted and Cell membrane.
  • Target information above from: UniProt accession O14788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)
    Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)

    ab129136 induced SEAP Production by RAW-blue TM cells.

  • Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)
    Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)

    ab129136 Induced production of TNF alpha from RAW cells.

  • SDS-PAGE - Recombinant mouse RANKL protein (Active) (ab129136)
    SDS-PAGE - Recombinant mouse RANKL protein (Active) (ab129136)

    1ug of ab129136 in a 4-20% Tris-Glycine gel, stained with Coomassie Blue.

    Lane 1: Reducing conditions.

    Lane 2: Non-reducing conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab129136? Please let us know so that we can cite the reference in this datasheet.

ab129136 has been referenced in 2 publications.

  • Liu X  et al. Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis. Nat Commun 12:1832 (2021). PubMed: 33758201
  • Elashiry M  et al. Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo. J Extracell Vesicles 9:1795362 (2020). PubMed: 32944183

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