Recombinant Mouse RELM Gamma protein (Animal Free) (Animal Free) (ab219127)

Description

  • Product name

    Recombinant Mouse RELM Gamma protein (Animal Free) (Animal Free)
    See all RELM Gamma proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    <=1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MEGTLESIVEKKVKELLANRDDCPSTVTKTFSCTSITASGRLASCPSGMT VTGCACGYGCGSWDIRDGNTCHCQCSTMDWATARCCQLA
    • Predicted molecular weight

      19 kDa
    • Amino acids

      24 to 111
    • Additional sequence information

      This product is the mature full length protein from aa 24 to 111. The signal peptide is not included. Dimer.
  • Description

    Recombinant Mouse RELM Gamma protein (Animal Free)

Specifications

Our Abpromise guarantee covers the use of ab219127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituent: 0.16% Sodium phosphate

    Lyophilized from a sterile 0.2 micron filtered solution.

  • Reconstitution
    Centrifuge vial before opening. Suspend the product by gently pipetting sterile deionized water down the sides of the vial to a final concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • Fizz3
    • Myeloid cysteine rich protein
    • Resistin like gamma
    • Resistin like molecule gamma
    • Retnlg
    • Xcp1
    see all
  • Relevance

    RELM gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Micro-array and real-time RT-PCR experiments revealed a repression of RELM gamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.
  • Cellular localization

    Secreted

Images

  • 1 µg ab219127 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.

    Lane 1: Non-reducing conditions.

    Lane 2: Reducing conditions.

    ab219127 is predicted to be a dimer with a total weight of 18.9 kDa, but the dimer runs larger (each monomer is predicted to be 9.4 kDa).

References

ab219127 has not yet been referenced specifically in any publications.

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