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    recombinant-mouse-scf-protein-animal-free-ab217435.pdf

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Immunology Innate Immunity Cytokines CSFs
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Recombinant mouse SCF protein (Animal Free) (ab217435)

  • Datasheet
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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, HPLC, Functional Studies

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Description

  • Product name

    Recombinant mouse SCF protein (Animal Free)
    See all SCF proteins and peptides
  • Biological activity

    The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 10.0 ng/mL, corresponding to a specific activity of ≥ 1 x 105 units/mg.

  • Purity

    > 98 % SDS-PAGE.
    > 98 % HPLC.
  • Expression system

    Escherichia coli
  • Accession

    P20826-1
  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
    • Predicted molecular weight

      18 kDa
    • Amino acids

      26 to 189
    • Additional sequence information

      This product is for the mature full length protein. The signal peptide is not included.

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab217435 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

      Functional Studies

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • C kit ligand
      • C-kit ligand
      • Ckit ligand
      • DCUA
      • DFNA69
      • DKFZp686F2250
      • familial progressive hyperpigmentation 2
      • FPH2
      • FPHH
      • KIT ligand
      • Kitl
      • KITLG
      • KL 1
      • KL1
      • Mast cell growth factor
      • MGF
      • MGF stem cell factor
      • SCF
      • SCF_HUMAN
      • SF
      • SHEP7
      • sKITLG
      • Soluble KIT ligand
      • Steel factor
      • steel, mouse, homolog of
      • Stem cell factor
      • Stem cell factor precursor
      see all
    • Function

      Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
    • Involvement in disease

      Hyperpigmentation with or without hypopigmentation, familial progressive
      Deafness, congenital, unilateral or asymmetric
    • Sequence similarities

      Belongs to the SCF family.
    • Developmental stage

      Acts in the early stages of hematopoiesis.
    • Post-translational
      modifications

      A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
      Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
      A soluble form exists as a cleavage product of the extracellular domain.
    • Cellular localization

      Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
    • Target information above from: UniProt accession P21583 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab217435? Please let us know so that we can cite the reference in this datasheet.

    ab217435 has not yet been referenced specifically in any publications.

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