Recombinant mouse SDF1 alpha protein (ab51939)
Key features and details
- Expression system: Escherichia coli
- Purity: > 99% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant mouse SDF1 alpha protein
See all SDF1 alpha proteins and peptides -
Biological activity
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.
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Purity
> 99 % SDS-PAGE.
>99% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg (1EU/µg). -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK -
Predicted molecular weight
8 kDa -
Amino acids
22 to 89 -
Additional sequence information
Mature full length for alpha isoform.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab51939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.06% Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionWe recommend a quick spin followed by reconstitution in sterile distilled water to a concentration of 0.1-1.0 mg/ml. (The protein may appear as a haze or film, which is difficult to see at the bottom of the vial.)
General Info
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Alternative names
- C-X-C motif chemokine 12
- Chemokine (C-X-C motif) ligand 12
- cxcl12
see all -
Function
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsProcessed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (2)
ab51939 has been referenced in 2 publications.
- Archacka K et al. Beneficial Effect of IL-4 and SDF-1 on Myogenic Potential of Mouse and Human Adipose Tissue-Derived Stromal Cells. Cells 9:N/A (2020). PubMed: 32560483
- Wu M et al. JAM-A promotes wound healing by enhancing both homing and secretory activities of mesenchymal stem cells. Clin Sci (Lond) 129:575-88 (2015). PubMed: 25994236