The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.
% SDS-PAGE. >99% by SDS-PAGE and HPLC analyses.
Endotoxin level is <0.1 ng per µg (1EU/µg).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.06% Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
We recommend a quick spin followed by reconstitution in sterile distilled water to a concentration of 0.1-1.0 mg/ml. (The protein may appear as a haze or film, which is difficult to see at the bottom of the vial.)
C-X-C motif chemokine 12
Chemokine (C-X-C motif) ligand 12
Intercrine reduced in hepatomas
Pre-B cell growth-stimulating factor
SDF 1 alpha
Stromal cell derived factor 1
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
Belongs to the intercrine alpha (chemokine CxC) family.
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.