Recombinant mouse TNF alpha protein (ab9740)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameRecombinant mouse TNF alpha protein
See all TNF alpha proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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Accession
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SpeciesMouse
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SequenceMLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
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Amino acids80 to 235
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab9740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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Applications
Functional Studies
SDS-PAGE
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FormLyophilised
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Additional notesThe ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
n/a
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionPlease reconstitute this product in 200ul water.
General Info
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Alternative names
- APC1
- APC1 protein
- Cachectin
see all -
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
Datasheets and documents
References
This product has been referenced in:
- Jensen P et al. TNFa affects CREB-mediated neuroprotective signaling pathways of synaptic plasticity in neurons as revealed by proteomics and phospho-proteomics. Oncotarget 8:60223-60242 (2017). Read more (PubMed: 28947966) »
