Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (ab219721)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active)
See all TNFSF18/GITRL proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse GITR, His Tag at 10μg/mL (100 μL/well) can bind ab219721 with a linear range of 0.007-0.256 μg/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
TAIESCMVKFELSS SKWHMTSPKP HCVNTTSDGK LKILQSGTYL IYGQVIPVDK KYIKDNAPFV VQIYKKNDVL QTLMNDFQIL PIGGVYELHA GDNIYLKFNS KDHIQKTNTY WGIILMPDLP FIS -
Predicted molecular weight
41 kDa including tags -
Amino acids
47 to 173 -
Additional sequence information
This protein carries a human IgG1 Fc tag at the N terminus. (Accession # AAO89011).
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Specifications
Our Abpromise guarantee covers the use of ab219721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride
Lyophilized from 0.22 µm filtered solution.
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- Activation inducible TNF related ligand
- Activation-inducible TNF-related ligand
- AITR ligand
see all -
Function
Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. -
Tissue specificity
Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
Images
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Immobilized Mouse GITR (His Tag) at 5 µg/mL (100 µL/well) can bind Mouse GITR Ligand (Fc Tag) with a linear range of 5-156 ng/mL (QC tested).
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SDS-PAGE analysis of ab219721 under reducing (lane 1) and non-reducing conditions (lane 2); stained overnight with Coomassie Blue.
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Immobilized Mouse GITR, His Tag at 10 μg/mL (100 μL/well) can bind ab219721 with a linear range of 0.007-0.256 μg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab219721 has not yet been referenced specifically in any publications.