Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, HPLC, ELISA
Product nameRecombinant mouse TRAP/CD40L protein (Active)
See all TRAP/CD40L proteins and peptides
Measured by its binding ability in a functional ELISA against immobilized Mouse CD40, Fc Tag (0.2 μg/well). The linear range is 9-78 ng/ml.
Purity> 95 % SDS-PAGE.
>95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceGDEDPQIAAHVVSEANSNAASVLQWAKKGYYTMKSNLVMLENGKQLTVKR EGLYYVYTQVTFCSNREPSSQRPFIVGLWLKPSSGSERILLKAANTHSSS QLCEQQSVHLGGVFELQAGASVFVNVTEASQVIHRVGFSSFGLLKL
Predicted molecular weight17 kDa including tags
Amino acids115 to 260
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab220551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
- CD 40L
- CD40 antigen ligand
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
SDS-PAGE analysis of ab220551 stained overnight with Coomassie Blue.
The reduced protein migrates as 21-25 kDa in SDS-PAGE.
Immobilized Mouse CD40, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220551 with a linear range of 9-78 ng/ml.
A SEC-HPLC analysis showing over 95% of ab220551 present as active trimers with a molecular mass of around 50-65 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab220551 has not yet been referenced specifically in any publications.