Recombinant mouse TRAP/CD40L protein (Soluble) (ab50160)

Description

  • Product name

    Recombinant mouse TRAP/CD40L protein (Soluble)
    See all TRAP/CD40L proteins and peptides
  • Biological activity

    Determined by its ability to induce TNF-α and MIP-1α production by murine splenocytes. The expected ED50 for this effect is < 0.1 µg/ml.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
    • Amino acids

      112 to 260
    • Additional sequence information

      Full length TRAP/CD40L, soluble form

Specifications

Our Abpromise guarantee covers the use of ab50160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names

    • CD 40L
    • CD154
    • CD40 antigen ligand
    • CD40 ligand
    • CD40 ligand, soluble form
    • CD40-L
    • CD40L
    • CD40L_HUMAN
    • CD40LG
    • gp39
    • hCD40L
    • HIGM1
    • IGM
    • IMD3
    • T B cell activating molecule
    • T BAM
    • T-cell antigen Gp39
    • TNF-related activation protein
    • TNFSF5
    • TrAP
    • Tumor necrosis factor (ligand) superfamily member 5
    • Tumor necrosis factor ligand superfamily member 5
    see all
  • Function

    Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificity

    Specifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in disease

    Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

References

ab50160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your enquiry. The bioassay for our Murine CD40 Ligand was determined using murine splenocytes to induce TNF-alpha and MIP-1 alpha. The ED50 for this is [0.1ug/ml] Please note that cytokines will react differently on different cell lines and conditions. There is also a reference on our website, where this cytokine has been used for dendritic cell maturation and activation. Please follow the hyperlink below for further details: http://www.jimmunol.org/cgi/content/full/174/5/2720 Please see the paragraph below referring to the method used: For dendritic cell maturation and activation, cells were cultured at 1 x 10^6/ml in 24-well plates, and stimulated with 500 ng/ml CD40L and 1 µg/ml LPS for 48 h. I hope this information has been of help, please do not hesitate to contact me should you have any additional questions.

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