Recombinant mouse UGDH protein (Active) (ab229177)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, MS
Description
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Product name
Recombinant mouse UGDH protein (Active)
See all UGDH proteins and peptides -
Biological activity
Specific activity is > 2,500 pmol/min/µg, and is defined as the amount of enzyme that convert 1.0 pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37ºC.
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Purity
> 90 % SDS-PAGE.
ab229177 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMVEIKKICCIGAGYVGGPTCSVIAHMC PEIRVTVVDVNEARINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDD AIREADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVT EKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDR VLIGGDETPEGQKAVRALCAVYEHWVPKEKILTTNTWSSELSKLAANAFL AQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCF QKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVT DKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIV VDLSHPGVSADDQVSRLVTISKDPYEACDGAHALVICTEWDMFKELDYER IHKKMLKPAFIFDGRRVLDGLHSELQTIGFQIETIGKKVSSKRIPYTPGE IPKFSLQDPPNKKPKV -
Predicted molecular weight
57 kDa including tags -
Amino acids
1 to 493 -
Tags
His tag N-Terminus -
Additional sequence information
NP_033492.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab229177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 5.00
Constituents: 0.39% MES, 20% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.03% EDTAThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- GDH
- UDP Glc dehydrogenase
- UDP GlcDH
see all -
Function
Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. -
Tissue specificity
Widely expressed. -
Pathway
Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. -
Sequence similarities
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229177 has not yet been referenced specifically in any publications.