Recombinant mouse Urokinase protein (ab92641)

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SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

    Key features and details

    • Expression system: Insect cells
    • Purity: > 98% SDS-PAGE
    • Active: Yes
    • Suitable for: ELISA, SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant mouse Urokinase protein
      See all Urokinase proteins and peptides

    • Biological activity

      The uPA activity of this product was tested using urokinase chromogenic substrate and standardize it to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. The specific activity of the last lot was 43,077 IU/mg.

    • Purity

      > 98 % SDS-PAGE.

    • Expression system

      Insect cells

    • Accession

      P06869

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mouse

      • Sequence

        GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF

      • Predicted molecular weight

        44 kDa

      • Amino acids

        21 to 433

      • Additional sequence information

        (Gene ID: 18792) Full length mature protein, without the signal peptide.

    Specifications

    Our Abpromise guarantee covers the use of ab92641 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Functional Studies

    • Form

      Liquid

    • Additional notes

      Spectrophotometric Data:

      Ultraviolet Absorbance (280nm) = 0.9

      epsilon 0.1% = 1.48

      ab92641 demonstrates preferential binding to mouse uPA over Human uPA coated on an ELISA plate.

      Produced using non-baculovirus insect cells.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 5.00
      Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • ATF
      • ATF uPA
      • BDPLT5
      • Plasminogen activator
      • Plasminogen activator urinary
      • Plasminogen activator urokinase
      • PLAU
      • QPD
      • u PA
      • U plasminogen activator
      • u-PA
      • U-plasminogen activator
      • uPA
      • URK
      • UROK_HUMAN
      • Urokinase plasminogen activator
      • Urokinase type plasminogen activator
      • Urokinase type plasminogen activator precursor
      • Urokinase-type plasminogen activator chain B
      see all

    • Function

      Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

    • Tissue specificity

      Expressed in the prostate gland and prostate cancers.

    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 EGF-like domain.
      Contains 1 kringle domain.
      Contains 1 peptidase S1 domain.

    • Post-translational
      modifications

      Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
      SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

      ab92641 on 12% SDS-PAGE.


      Lane 1: 5 ug, reduced. 
      Lane 2: + CPAI, 20 ug, reduced.
      Lane 3: 5 ug, non-reduced.
      Lane 4: + CPAI, 20 ug, non-reduced.
      Lane 5: Prestained Standard

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab92641? Please let us know so that we can cite the reference in this datasheet.

    ab92641 has been referenced in 1 publication.

    • Semina EV  et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070

    Customer reviews and Q&As

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    Question

    ab92727
    ab92641
    Please provide the ELISA protocol

    Read More
    Answer



    For ab92641, I contacted the laboratories. They confirmed they used a 1 ug/ml solution of MUPA in TBS or PBS. Apply to ELISA wells and incubate at 4 C overnight. Then block the plates with a commercial blocking reagent according to the manufacturer’s protocol, washing if required. Then antibodies to mouse uPA can be applied as desired followed by an appropriate secondary.

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