Recombinant mouse Urokinase protein (ab92641)
Key features and details
- Expression system: Insect cells
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant mouse Urokinase protein
See all Urokinase proteins and peptides -
Biological activity
The uPA activity of this product was tested using urokinase chromogenic substrate and standardized to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. If you need the specific activity value please contact us with the lot# you require the data for.
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Purity
> 98 % SDS-PAGE. -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF -
Predicted molecular weight
44 kDa -
Amino acids
21 to 433 -
Additional sequence information
(Gene ID: 18792) Full length mature protein, without the signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab92641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Spectrophotometric Data:
Ultraviolet Absorbance (280nm) = 0.9
epsilon 0.1% = 1.48
ab92641 demonstrates preferential binding to mouse uPAR over Human uPAR coated on an ELISA plate.
Produced using non-baculovirus insect cells.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 5.00
Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all -
Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a higher molecular weight.
The observed band at 44 kDa in lane 1 corresponds to mouse Urokinase protein under non-reducing conditions.
Lane 1: 3 µg mouse urokinase protein, non-reduced.
Lane 2: + CPAI, 10 µg, non-reduced.Lane 3: Molecular Weight Markers
Lane 4: 3 µg mouse urokinase protein, reduced.
Lane 5: + CPAI, 10 µg, reduced. -
SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a high molecular weight.
The observed band at 44 kDa in lane 3 corresponds to mouse Urokinase protein under non-reducing conditions.
Lane 1: 5 µg mouse urokinase protein, reduced.
Lane 2: + CPAI, 20 ug, reduced.
Lane 3: 5 ug mouse urokinase protein, non-reduced.
Lane 4: + CPAI, 20 ug, non-reduced.
Lane 5: Prestained Standard Ladder
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92641 has been referenced in 1 publication.
- Semina EV et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070