Recombinant mouse Urokinase protein (ab92641)

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Description

  • Product name

    Recombinant mouse Urokinase protein
    See all Urokinase proteins and peptides

  • Biological activity

    The uPA activity of this product was tested using urokinase chromogenic substrate and standardize it to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. The specific activity of the last lot was 43,077 IU/mg.

  • Purity

    > 98 % SDS-PAGE.

  • Expression system

    Insect cells

  • Accession

    P06869

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF

    • Predicted molecular weight

      44 kDa

    • Amino acids

      21 to 433

    • Additional sequence information

      (Gene ID: 18792) Full length mature protein, without the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab92641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Functional Studies

  • Form

    Liquid

  • Additional notes

    Spectrophotometric Data:

    Ultraviolet Absorbance (280nm) = 0.9

    epsilon 0.1% = 1.48

    ab92641 demonstrates preferential binding to mouse uPA over Human uPA coated on an ELISA plate.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 5.00
    Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ATF
    • ATF uPA
    • BDPLT5
    • Plasminogen activator
    • Plasminogen activator urinary
    • Plasminogen activator urokinase
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    • Urokinase type plasminogen activator precursor
    • Urokinase-type plasminogen activator chain B
    see all

  • Function

    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

  • Tissue specificity

    Expressed in the prostate gland and prostate cancers.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
    SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

    ab92641 on 12% SDS-PAGE.


    Lane 1: 5 ug, reduced. 
    Lane 2: + CPAI, 20 ug, reduced.
    Lane 3: 5 ug, non-reduced.
    Lane 4: + CPAI, 20 ug, non-reduced.
    Lane 5: Prestained Standard

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab92641? Please let us know so that we can cite the reference in this datasheet.

ab92641 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

ab92727
ab92641
Please provide the ELISA protocol

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Answer



For ab92641, I contacted the laboratories. They confirmed they used a 1 ug/ml solution of MUPA in TBS or PBS. Apply to ELISA wells and incubate at 4 C overnight. Then block the plates with a commercial blocking reagent according to the manufacturer’s protocol, washing if required. Then antibodies to mouse uPA can be applied as desired followed by an appropriate secondary.

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