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    recombinant-mouse-urokinase-protein-ab92641.pdf

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Recombinant mouse Urokinase protein (ab92641)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
  • SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

Key features and details

  • Expression system: Insect cells
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: ELISA, SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant mouse Urokinase protein
    See all Urokinase proteins and peptides
  • Biological activity

    The uPA activity of this product was tested using urokinase chromogenic substrate and standardized to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. If you need the specific activity value please contact us with the lot# you require the data for.

  • Purity

    > 98 % SDS-PAGE.

  • Expression system

    Insect cells
  • Accession

    P06869
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF
    • Predicted molecular weight

      44 kDa
    • Amino acids

      21 to 433
    • Additional sequence information

      (Gene ID: 18792) Full length mature protein, without the signal peptide.

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-Urokinase antibody [H77A10] (ab28230)
    • Anti-6X His tag® antibody [4D11] (ab5000)

Specifications

Our Abpromise guarantee covers the use of ab92641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    Spectrophotometric Data:

    Ultraviolet Absorbance (280nm) = 0.9

    epsilon 0.1% = 1.48

    ab92641 demonstrates preferential binding to mouse uPAR over Human uPAR coated on an ELISA plate.

    Produced using non-baculovirus insect cells.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 5.00
    Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ATF
    • ATF uPA
    • BDPLT5
    • Plasminogen activator
    • Plasminogen activator urinary
    • Plasminogen activator urokinase
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    • Urokinase type plasminogen activator precursor
    • Urokinase-type plasminogen activator chain B
    see all
  • Function

    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificity

    Expressed in the prostate gland and prostate cancers.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P00749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
    SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

    SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a higher molecular weight.

    The observed band at 44 kDa in lane 1 corresponds to mouse Urokinase protein under non-reducing conditions.


    Lane 1: 3 µg mouse urokinase protein, non-reduced. 
    Lane 2: + CPAI, 10 µg, non-reduced.

    Lane 3: Molecular Weight Markers

    Lane 4: 3 µg mouse urokinase protein, reduced.
    Lane 5: + CPAI, 10 µg, reduced.


  • SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
    SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

    SDS-PAGE analysis of mouse urokinase protein (ab92641) under reducing and non-reducing conditions in 12% SDS-PAGE. The urokinase protein is demonstrated to be active through the observed binding to Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein (ab92969) (CPAI protein). CPAI is an inhibitor of urokinase, mutated to be more stable, and the two form a complex that migrates in the gel to a high molecular weight.

    The observed band at 44 kDa in lane 3 corresponds to mouse Urokinase protein under non-reducing conditions.


    Lane 1: 5 µg mouse urokinase protein, reduced. 
    Lane 2: + CPAI, 20 ug, reduced.

    Lane 3: 5 ug mouse urokinase protein, non-reduced.
    Lane 4: + CPAI, 20 ug, non-reduced.

    Lane 5: Prestained Standard Ladder

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab92641? Please let us know so that we can cite the reference in this datasheet.

ab92641 has been referenced in 1 publication.

  • Semina EV  et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070

Customer reviews and Q&As

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Question

ab92727
ab92641
Please provide the ELISA protocol

Read More

Abcam community

Verified customer

Asked on Aug 28 2015

Answer



For ab92641, I contacted the laboratories. They confirmed they used a 1 ug/ml solution of MUPA in TBS or PBS. Apply to ELISA wells and incubate at 4 C overnight. Then block the plates with a commercial blocking reagent according to the manufacturer’s protocol, washing if required. Then antibodies to mouse uPA can be applied as desired followed by an appropriate secondary.

Read More

Sam Washer

Abcam Scientific Support

Answered on Aug 28 2015

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