Recombinant Mouse Urokinase protein (ab92641)

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Overview

Description

  • Nature

    Recombinant

  • Source

    Insect cells
  • Amino Acid Sequence
    • Accession
      P06869

    • Species

      Mouse

    • Sequence

      GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF

    • Molecular weight

      44 kDa

    • Amino acids

      21 to 433

    • Additional sequence information

      (Gene ID: 18792) Full length mature protein, without the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab92641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    ELISA

    SDS-PAGE

  • Purity

    > 98 % SDS-PAGE.

  • Form

    Liquid

  • Additional notes

    Spectrophotometric Data:

    Ultraviolet Absorbance (280nm) = 0.9

    epsilon 0.1% = 1.48

    ab92641 demonstrates preferential binding to mouse uPA over Human uPA coated on an ELISA plate.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 5.00
    Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chloride

General Info

  • Alternative names

    • ATF
    • ATF uPA
    • BDPLT5
    • Plasminogen activator
    • Plasminogen activator urinary
    • Plasminogen activator urokinase
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    • Urokinase type plasminogen activator precursor
    • Urokinase-type plasminogen activator chain B
    see all

  • Function

    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

  • Tissue specificity

    Expressed in the prostate gland and prostate cancers.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)
    SDS-PAGE - Recombinant mouse Urokinase protein (ab92641)

    ab92641 on 12% SDS-PAGE.


    Lane 1: 5 ug, reduced. 
    Lane 2: + CPAI, 20 ug, reduced.
    Lane 3: 5 ug, non-reduced.
    Lane 4: + CPAI, 20 ug, non-reduced.
    Lane 5: Prestained Standard

References

ab92641 has not yet been referenced specifically in any publications.

Publishing research using ab92641? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

ab92727
ab92641
Please provide the ELISA protocol

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Answer



For ab92641, I contacted the laboratories. They confirmed they used a 1 ug/ml solution of MUPA in TBS or PBS. Apply to ELISA wells and incubate at 4 C overnight. Then block the plates with a commercial blocking reagent according to the manufacturer’s protocol, washing if required. Then antibodies to mouse uPA can be applied as desired followed by an appropriate secondary.

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