Key features and details
- Expression system: Insect cells
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies
Product nameRecombinant mouse Urokinase protein
See all Urokinase proteins and peptides
The uPA activity of this product was tested using urokinase chromogenic substrate and standardize it to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. The specific activity of the last lot was 43,077 IU/mg.
Purity> 98 % SDS-PAGE.
Expression systemInsect cells
Protein lengthFull length protein
SequenceGSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF
Predicted molecular weight44 kDa
Amino acids21 to 433
Additional sequence information(Gene ID: 18792) Full length mature protein, without the signal peptide.
Our Abpromise guarantee covers the use of ab92641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Ultraviolet Absorbance (280nm) = 0.9
epsilon 0.1% = 1.48
ab92641 demonstrates preferential binding to mouse uPA over Human uPA coated on an ELISA plate.
Produced using non-baculovirus insect cells.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.41% Sodium acetate, 0.0292% EDTA, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ATF uPA
FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Tissue specificityExpressed in the prostate gland and prostate cancers.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab92641 has been referenced in 1 publication.
- Semina EV et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070