We treated our cells for 24 hours with 200 ng/mL Wnt3a and then examined Wnt/beta-catenin target gene (e.g. Axin2) expression by qRT-PCR or Tcf/Lef activity by using a dual-luciferase reporter system (Super 8X-TOP/FOP-Flash).
The Wnt3a-mediated activation of the Wnt/beta-catenin pathway, as assessed by target gene activation or Tcf/Lef reporter activity, was not appreciably different from that observed when recombinant Wnt3a from other vendors was used.
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Submitted Dec 05 2014