High-quality recombinant Wnt3a

Functional Studies
This Wnt3a was used to treat mouse embryonic stem cells, which are quite responsive to Wnt3a.
We treated our cells for 24 hours with 200 ng/mL Wnt3a and then examined Wnt/beta-catenin target gene (e.g. Axin2) expression by qRT-PCR or Tcf/Lef activity by using a dual-luciferase reporter system (Super 8X-TOP/FOP-Flash).

The Wnt3a-mediated activation of the Wnt/beta-catenin pathway, as assessed by target gene activation or Tcf/Lef reporter activity, was not appreciably different from that observed when recombinant Wnt3a from other vendors was used.

Abcam user community

Verified customer

Submitted Dec 05 2014

Sign up