Description

  • Product name

    Recombinant Mycobacterium tuberculosis Ag85B protein
    See all Mycobacterium tuberculosis Ag85B proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    Endotoxin Tested
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Sequence

      MTDVSRKIRA WGRRLMIGTA AAVVLPGLVG LAGGAATAGA FSRPGLPVEY LQVPSPSMGR DIKVQFQSGG NNSPAVYLLD GLRAQDDYNG WDINTPAFEW YYQSGLSIVM PVGGQSSFYS DWYSPACGKA GCQTYKWETF LTSELPQWLS ANRAVKPTGS AAIGLSMAGS SAMILAAYHP QQFIYAGSLS ALLDPSQGMG PSLIGLAMGD AGGYKAADMW GPSSDPAWER NDPTQQIPKL VANNTRLWVY CGNGTPNELG GANIPAEFLE NFVRSSNLKF QDAYNAAGGH NAVFNFPPNG THSWEYWGAQ LNAMKGDLQS SLGAG
    • Predicted molecular weight

      30 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab83471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 7.50
    Constituents: 0.24% Monobasic dihydrogen sodium phosphate, 0.284% Dibasic monohydrogen sodium phosphate, 0.328% Sodium phosphate, 10% Glycerol, 2.9% Sodium chloride

  • Reconstitution
    ab83471 should be reconstituted in apirogenic water or PBS and has to be used immediately

General Info

  • Alternative names

    • 30 kDa extracellular protein
    • Antigen 85 complex B
    • Mycobacterium
    • Mycolyl transferase 85B
  • Relevance

    Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall.

References

ab83471 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.
Please find below the answers to your questions:

1.
I am happy to confirm that this protein (ab124604)is Antigen 85 sub unit A.

2.
a) ab73632 is the full length protein (aa 1 to 325)
b) I can confirm that ab833471 is also un-glycosylated because it was produced in E. coli which is missing the glycosylation machinery of eukaryotic cells.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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