Recombinant Mycobacterium tuberculosis Ag85 protein (His tag) (ab225596)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Mycobacterium tuberculosis Ag85 protein (His tag)
See all Mycobacterium tuberculosis Ag85 proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mycobacterium tuberculosis -
Sequence
AFSRPGLPVEYLQVPSASMGRDIKVQFQGGGPHAVYLLDGLRAQDDYNGW DINTPAFEEYYQSGLSVIMPVGGQSSFYTDWYQPSQSNGQNYTYKWETFL TREMPAWLQANKGVSPTGNAAVGLSMSGGSALILAAYYPQQFPYAASLSG FLNPSEGWWPTLIGLAMNDSGGYNANSMWGPSSDPAWKRNDPMVQIPRLV ANNTRIWVYCGNGTPSDLGGDNIPAKFLEGLTLRTNQTFRDTYAADGGRN GVFNFPPNGTHSWPYWNEQLVAMKADIQHVLNGATPPAAPAAPAA -
Predicted molecular weight
36 kDa including tags -
Amino acids
46 to 340 -
Tags
His tag N-Terminus -
Additional sequence information
This product is the mature full length protein from aa 46 to 340. The signal peptide is not included. Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
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Description
Recombinant Mycobacterium tuberculosis Mycobacterium tuberculosis Ag85 protein (His tag)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab225596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer
General Info
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Alternative names
- Antigen 85 complex A
- Antigen 85 complex B
- Antigen 85 complex C
see all -
Relevance
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225596 with 5% enrichment gel and 15% separation gel.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225596 could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) Ag85.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225596 could indicate that this peptide derived from E.coli-expressed Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) Ag85.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225596 has not yet been referenced specifically in any publications.