Recombinant OPRT protein (Tagged) (ab240863)

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SDS-PAGE - Recombinant OPRT protein (Tagged) (ab240863)

    Key features and details

    • Expression system: Yeast
    • Purity: > 90% SDS-PAGE
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant OPRT protein (Tagged)
      See all OPRT proteins and peptides

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Yeast

    • Accession

      P0CS95

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Sequence

        MSSQALDSAKVAFIEAAIEHGVLLFGNFTLKSGRQSPYFFNAGLLYSSSL LSTTAQAYAKVLSSSRIPDFDVLFGPAYKGISLAAVSAVSLYQQTGKDIG YCYNRKEKKDHGEGGTMVGAPLKGRIVIIDDVLTSGKAIREAIDILKASP EAKLVGIVQLVDRQEKGQSGSGKSTVQEVEEEFGVPVEPIIGLDDIVKYL ESSGKWEKELQEVRKYRAEYGVQRS

      • Predicted molecular weight

        28 kDa including tags

      • Amino acids

        1 to 225

      • Additional sequence information

        Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans). N-terminal 10xHis-tagged and C-terminal Myc-tagged.

    Specifications

    Our Abpromise guarantee covers the use of ab240863 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as UMPS

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • ODC
      • OMPdecase
      • OPRT
      • OPRTase
      • Orotate phosphoribosyl transferase and orotidine 5' decarboxylase
      • Orotate phosphoribosyltransferase
      • Orotate phosphoribosyltransferase phosphoribosyltransferase
      • Orotidine 5' phosphate decarboxylase
      • Orotidine 5''-phosphate decarboxylase
      • RP11-71H17.9
      • UMP synthase
      • Umps
      • UMPS_HUMAN
      • Uridine 5' monophosphate synthase
      • Uridine monophosphate synthetase
      see all

    • Pathway

      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

    • Involvement in disease

      Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.

    • Sequence similarities

      In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
      In the C-terminal section; belongs to the OMP decarboxylase family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant OPRT protein (Tagged) (ab240863)
      SDS-PAGE - Recombinant OPRT protein (Tagged) (ab240863)

      (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab240863.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240863? Please let us know so that we can cite the reference in this datasheet.

    ab240863 has not yet been referenced specifically in any publications.

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