Recombinant Paramyxovirus SV5 Pk protein (ab204207)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant Paramyxovirus SV5 Pk protein

  • Purity

    > 90 % SDS-PAGE.
    This protein was expressed in E. coli as inclusion bodies. The final product was refolded and chromatographically purified.

  • Expression system

    Escherichia coli

  • Accession

    P11207

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Parainfluenza virus 5

    • Sequence

      MASMTGGQQMGRGHHHHHHENLYFQGDPTDLSFSPDEINKLIETGLNTVE YFTSQQVTGTSSLGKNTIPPGVTGLLTNAAEAKIQESTNHQKGSVGGGAK PKKPRPKIAIVPADDKTVPGKPIPNPLLGLDSTPSTQTVLDLSGKTLPSG SYKGVKLAKFGKENLMTRFIEEPRENPIATSSPIDFKRGRDTGGFHRREY SIGWVGDEVKVTEWCNPSCSPITAAARRFECTCHQCPVTCSECERDTESG GGGSPGRRRRRRRRRRR

    • Predicted molecular weight

      29 kDa including tags

    • Amino acids

      2 to 222

    • Additional sequence information

      from Parainfluenza virus 5, and with small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal, and a 11 arginine (11R) tag was added at its C-terminal.

Specifications

Our Abpromise guarantee covers the use of ab204207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituent: 0.32% Tris HCl

    Contains NaCl, KCl, EDTA, Sucrose and DTT.

General Info

  • Alternative names

    • Simian virus 5
    • SPV5gp2
    • V protein

  • Relevance

    The paramyxovirus family includes many well-known human and animal pathogens as well as emerging viruses such as Hendra virus and Nipah virus. The V protein of simian virus 5 (SV5), a prototype of the paramyxoviruses, contains a cysteine-rich C-terminal domain which is conserved among all paramyxovirus V proteins. The V protein can block both interferon (IFN) signaling by causing degradation of STAT1 and IFN production by blocking IRF-3 nuclear import.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab204207? Please let us know so that we can cite the reference in this datasheet.

ab204207 has not yet been referenced specifically in any publications.

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