Recombinant Pig IL-22 protein (ab101291)

Submit a review Q&A (2)

Key features and details

  • Expression system: Yeast
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Pig IL-22 protein
    See all IL-22 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab101291 was purified by ion-exchange chromatography

  • Expression system

    Yeast

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Pig

    • Sequence

      VPITHHCKLD QSNFQQPYIT NRTFTLAQEA SLADNNTDVR LIGNNLFQGV NMRERCYLVK QVLNFTLEEV LFPNSDRFHP YMQEVASFLD SLSKKLSQCR IKGDDQHIQR NVNNFKDTVK KLGESGEIKV IGELYLLFMA LKNECTLPGH SWKMDN

    • Predicted molecular weight

      18 kDa

Specifications

Our Abpromise guarantee covers the use of ab101291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituents: 2.68% PBS, 10% Trehalose

  • Reconstitution
    Reconstitute with sterile PBS containing at least 0.1% carrier protein (BSA)

General Info

  • Alternative names

    • Cytokine Zcyto18
    • IL 10 related T cell derived inducible factor
    • IL 21
    • IL 22
    • IL D110
    • IL TIF
    • IL-10-related T-cell-derived-inducible factor
    • IL-22
    • IL-TIF
    • IL21
    • Il22
    • IL22_HUMAN
    • ILD110
    • ILTIF
    • Interleukin 10 related T cell derived inducible factor
    • interleukin 21
    • Interleukin 22
    • Interleukin-22
    • MGC79382
    • MGC79384
    • TIFa
    • TIFIL 23
    • TIFIL23
    • UNQ3099/PRO10096
    • zcyto18
    see all

  • Function

    Cytokine that contributes to the inflammatory response in vivo.

  • Sequence similarities

    Belongs to the IL-10 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab101291? Please let us know so that we can cite the reference in this datasheet.

ab101291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

thank you for your quick response. Regarding IL-24 I have additional questions. I am looking for a solution to control IL-17Ahistagged, IL-17Fhistagged would be the best option. As I understood you informed me IL-24 is an alias name for IL-17F. However I am not able to find proof it is the same gene (on for example NCBI-gene).

Can you try to follow-up on a solution for IL-17F histagged?

Moreover I discovered another product (ab40044). Some questions:

why are there some residues of the signal peptide included -starts at residue 20 instead of 24-?

why this one is so much cheaper than the ab82830?

I realize the histagg is on the C-terminus. This can actually be an interesting control.

Kind regards,

Read More
Answer

Thank you for contacting us.

Please check the following link http://www.uniprot.org/uniprot/Q96PD4; IL24 is synonym for IL17F as given in uniprot protein database however the uniprot ID and GeneID for both proteins are different. I am sorry I am unsure why the name used as synonym but different IDS. You may need to search this in literature. The following are the useful links;

http://www.uniprot.org/uniprot/Q13007

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11009

http://www.uniprot.org/uniprot/Q96PD4

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=112744

The IL17F protein we have are

https://www.abcam.com/IL17F-protein-Human-ab78475.html
https://www.abcam.com/IL17F-protein-Active-ab119722.html
https://www.abcam.com/IL17F-protein-ab50157.html (this is homo-dimer)
https://www.abcam.com/IL17F-protein-Active-ab85600.html

The protein ab40044 is not IL17F it is actualy IL17A or IL17; http://www.uniprot.org/uniprot/Q16552

I have asked lab to confirm, why the signal peptide amino acids are included. I will send you the details soon.

These products are developed by our collaborators and they are from different suppliers which is why the price is different.

Finally, we can provide the product at ab82830 at 0.5mg/ml which is the lower limit at which the product can be used with conjugation kits.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

What is the sequence of IL-17 protein?. Does the sequence have signal peptide?
Do you have IL24 and IL22 proteins available?
Could I get these protein HIs tagged as well?
Is the product ab82830 in homodimer or dimerize with IL17F?
Could we get the product conjugated to FITC or PerCP/Cy5.5

Read More
Answer

Thank you for your phone call.

The protein ab82830 is a monomer; it not homodimer. The amino acid sequence can be found at http://www.uniprot.org with protein ID Q16552.

We unfortunately will not be able to supply this protein as FITC conjugated or PerCP conjugated product. I am sorry our lab will not be able to conjugate these on custom basis. I however have requested lab if they can provide this protein in higher concentration so that the conjugation kits can be used.

We will not be able to provide ab49821, IL22 protein as His tagged.

The IL24 protein we have is ab131696.

I will let you know further when I receive a reply form lab.

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