For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Our Abpromise guarantee covers the use of ab52953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. . The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Endotoxin level is <0.1 EU/mg of Protein A.
ab52953 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"