• Nature
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Molecular weight
      39 kDa
    • Amino acids
      32 to 327
    • Tags
      His tag N-Terminus
    • Additional sequence information
      Staphylococcus aureus subsp.aureus. Protein A Ig binding domains with 6x His-tag on N-terminus


Our Abpromise guarantee covers the use of ab52953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Endotoxin level
    < 0.100 Eu/mg
  • Purity
    > 95 % SDS-PAGE.
    Purity: >99% by SDS-PAGE and HPLC analyses.
  • Form
  • Additional notes

    The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. . The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. 

    Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).

    It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
    Protein A has little affinity to chicken, goat, rat and sheep. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: dH2O
    Endotoxin level is <0.1 EU/mg of Protein A.

General Info

  • Alternative names
    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A
  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.


ab52953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en compl&ea...

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Je viens d'être informé que:

1) les deux protéines A et G His-taggées ab49807 et ab52953 ne sont malheureusement pas disponibles dans la forme sans tag.

2) nous avons ou pouvons ajouter à notre catalo...

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Je suis toujours en attente d'une réponse pour une proposition de protéine His-tag pouvant fixer le fer ou un métal lourd.

Concernant les protéines A et G, nous avons ab49807 (protéine G, acides aminés...

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Thank you for your enquiry. I can confirm that the molecular weight of the protein and His tag is 39kDa. Unfortunately, we do not have further information regarding the diameter, nor the affinity constant. However, we found that binding to human Ig...

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