Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Molecular weight

      31 kDa
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Contains only one single cysteine at the C-terminus, which is designed for single point attachment coupling for immobilization.

Specifications

Our Abpromise guarantee covers the use of ab155724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 98 % SDS-PAGE.
    ab155724 was lyophilized from 0.22 µm filtered solution.
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

General Info

  • Alternative names

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • Relevance

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Cell Wall and Secreted

Images

  • SDS-PAGE analysis of reduced ab155724 stained overnight with Coomassie Blue.

  • Immobilized Recombinant Protein G, His Tag (ab155724) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (ab246197) with a linear range of 0.01-0.313 μg/mL

References

ab155724 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer



Just like the Protein G, our Protein A protein (His tag) ab155695 contains only one C terminus cysteine.This product , Protein A , contains 4 binding sites to IgG, and ab155724 Protein G protein (His tag) contains 2 binding sites.

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Question
Answer

This recombinant Protein G contains totally only one single cysteine at the C-terminus, which is designed for single point attachment coupling for immobilization.

Read More

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