Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Molecular weight
      26 kDa including tags
    • Amino acids
      190 to 384
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab49807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Affinity purification

  • Purity
    > 95 % SDS-PAGE.
    >98% by SDS-PAGE and HPLC analyses. The albumin binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
  • Form
    Lyophilised
  • Additional notes


    Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies.

    This protein contains only IgG binding domains.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is <0.1 EU/mg of Protein G.

General Info

  • Alternative names
    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • Relevance
    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Cell Wall and Secreted

References

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab49807? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
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What liquid is this protein stored in?

The protein is resuspended in water to a concentration of 5 mg/ml.

ab7248 Anti-Protein G antibody
ab33110 Goat F(ab) polyclonal Secondary Antibody to Rabbit IgG (1.4nm Gold (ultra-small))
ab49807 Protein G protein (His tag)
ab73758 Protein G protein



Ma collègue est actuellement en contact avec un laboratoire américain pour la protéine G entière sans tag.

Elle m'a dit qu'elle espérait un retour dans la semaine mais n'oublions pas que jeudi et ve...

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- protein G (full length)
- protein G (full length) + His tag ....... ab7458.

- protein A (full length) ....... ab117657.
- protein A (full length) + His tag

J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en compl&ea...

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protéines pouvant fixer les métaux

Je viens d'être informé que:

1) les deux protéines A et G His-taggées ab49807 et ab52953 ne sont malheureusement pas disponibles dans la forme sans tag.

2) nous avons ou pouvons ajouter à notre catalo...

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ab96301 disponible ou pas?
Séquence de ab49807. Phosphate détecté, protéine phosphorylée ou pas? Tampon de stockage?
Proteine His-tag fixant le fer ou métal lourd.
Proteine A et G avec et sans His-tag.

Je suis toujours en attente d'une réponse pour une proposition de protéine His-tag pouvant fixer le fer ou un métal lourd.

Concernant les protéines A et G, nous avons ab49807 (protéine G, acides aminés...

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ab96301 disponible ou pas?
Séquence de ab49807. Phosphate détecté, protéine phosphorylée ou pas? Tampon de stockage?

Voici comme convenu la réponse du laboratoire ainsi que le séquence de ab49807:

ab49807 : EL DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLK...

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The 17 kDa product would not seem to be quite large enough even for just the 3 IgG binding domains (˜195 AA), but anyway I’m interested in one of the other protein G catalog items.
The information you provided about quality systems doesn&rsq...

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I was forwarded your information by my colleague, and I'm happy to help out with your enquiries.

I'll be in touch with the manufacturing lab, and I can give you a call anytime this afternoon. Are you free around noon CDT?

Please let...

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