Recombinant Protein G (ab49807)

Submit a review Q&A (7)References (4)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus

Description

  • Product name

    Recombinant Protein G
    See all Protein G proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    >98% by SDS-PAGE and HPLC analyses. The albumin binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.

  • Expression system

    Escherichia coli

  • Accession

    P19909

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Streptococcus

    • Predicted molecular weight

      26 kDa including tags

    • Amino acids

      190 to 384

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab49807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Affinity Purification

  • Form

    Lyophilized

  • Additional notes


    Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies.

    This protein contains only IgG binding domains.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Info

  • Alternative names

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all

  • Relevance

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

  • Cellular localization

    Cell Wall and Secreted

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab49807? Please let us know so that we can cite the reference in this datasheet.

ab49807 has been referenced in 4 publications.

  • Korodi M  et al. Reusable on-plate immunoprecipitation method with covalently immobilized antibodies on a protein G covered microtiter plate. J Immunol Methods 483:112812 (2020). PubMed: 32569597
  • Park S  et al. Force-dependent trans-endocytosis by breast cancer cells depletes costimulatory receptor CD80 and attenuates T cell activation. Biosens Bioelectron 165:112389 (2020). PubMed: 32729511
  • Li IT  et al. Mapping cell surface adhesion by rotation tracking and adhesion footprinting. Sci Rep 7:44502 (2017). PubMed: 28290531
  • Wang X  et al. Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors. Sci Rep 6:21584 (2016). PubMed: 26875524

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Question

What liquid is this protein stored in?

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Answer

The protein is resuspended in water to a concentration of 5 mg/ml.

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Question

ab7248 Anti-Protein G antibody
ab33110 Goat F(ab) polyclonal Secondary Antibody to Rabbit IgG (1.4nm Gold (ultra-small))
ab49807 Protein G protein (His tag)
ab73758 Protein G protein

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Answer



Ma collègue est actuellement en contact avec un laboratoire américain pour la protéine G entière sans tag.

Elle m'a dit qu'elle espérait un retour dans la semaine mais n'oublions pas que jeudi et vendredi les américains fêtent Thanksgiving, ce qui pourrait retarder le processus.

Je vous fais parvenir dans un autre email un devis avec les produits dont nous avons parlé la semaine dernière.

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Question

- protein G (full length)
- protein G (full length) + His tag ....... ab7458.

- protein A (full length) ....... ab117657.
- protein A (full length) + His tag

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Answer

J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en complément de la référence ab7458 (protéine G, full length, His tag),
- une protéine A (full length avec His-tag) en complément de la référence ab117657 (protéine A, full length, sans tag)

Je vous contacterai de nouveau avec les informations que mes collègues m'auront fournies.

Merci pour votre patience et bon week-end.

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Question

protéines pouvant fixer les métaux

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Answer

Je viens d'être informé que:

1) les deux protéines A et G His-taggées ab49807 et ab52953 ne sont malheureusement pas disponibles dans la forme sans tag.

2) nous avons ou pouvons ajouter à notre catalogue les protéines His-tag ci-dessous, qui je l'espère, correspondent à vos besoins. Le collaborateur qui m'a aidé à générer cette liste m'a indiqué que la capacité de ces protéines à fixer le fer ou les métaux lourds n'a pas encore été déterminée.

Cobalt:
ab124578 - Methionyl Aminopeptidase 1 protein (His tag), 1-386aa, Human, https://www.abcam.com/ab124578
ab106876 - map protein (His tag), 1-264aa, E.coli, https://www.abcam.com/ab106876

Cuivre:
ab111633 - CUTC protein, 1-273aa, Human, His tag, https://www.abcam.com/ab111633
ATOX1, 1-68aa, Human, His tag, E.coli. Peut être ajoutée au catalogue sur demande.

Zinc:
msrB, 1-137aa, E.coli, His tag. Peut être ajoutée au catalogue sur demande.
ab89359 - Carbonic Anhydrase I protein (His tag), 1-261aa, Human, https://www.abcam.com/ab89359
ab131675 - TIMP2 protein (His tag), 27-220aa, Human, https://www.abcam.com/ab131675
ab139776 - STAMBPL1 protein (His tag), 1-436aa Human, https://www.abcam.com/ab139776

Divalent metal cations:
ab104015 - FAHD1 protein, 1-224aa, Human, His tag, https://www.abcam.com/ab104015
ab126654 - TATDN3 protein (His tag), 1-274aa, Human, https://www.abcam.com/ab126654

Fer:
ab139782 - Hemoglobin subunit gamma 2 protein (His tag), 1-147aa Human, https://www.abcam.com/ab139782
ab95347 - HBZ protein (His tag), 1-142aa, Human, https://www.abcam.com/ab95347

Fer ou Nickel:
ab140543 - ADI1 protein (His tag), 1-179aa, Human, https://www.abcam.com/ab140543


J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Bonne fin de semaine et bon week-end.

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Question

ab96301 disponible ou pas?
Séquence de ab49807. Phosphate détecté, protéine phosphorylée ou pas? Tampon de stockage?
Proteine His-tag fixant le fer ou métal lourd.
Proteine A et G avec et sans His-tag.

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Answer

Je suis toujours en attente d'une réponse pour une proposition de protéine His-tag pouvant fixer le fer ou un métal lourd.

Concernant les protéines A et G, nous avons ab49807 (protéine G, acides aminés 189-384 + 6xHis-tag) et ab52953 (protéine A, acides aminés 32-327 + 6xHis-tag). Malheureusement nous n'avons pas pour l'instant les équivalents exacts de ces 2 recombinantes sans His-tag. Je suis également en attente de savoir si il y a possibilité d'obtenir ces 2 fragments sans les 6xHis-tag.

Je vous transmettrai ces informations dès que possible. Merci pour votre patience.

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Question

ab96301 disponible ou pas?
Séquence de ab49807. Phosphate détecté, protéine phosphorylée ou pas? Tampon de stockage?

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Answer

Voici comme convenu la réponse du laboratoire ainsi que le séquence de ab49807:

ab49807 : EL DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLK

Cette protéine est bien produite chez E.Coli.

Le laboratoire a ajouté le commentaire suivant:

"This protein is lyophilized without any additives and reconstituted in water. There is no other storage buffer involved. This protein is >98% pure. The remaining part which is impure might contain some phosphate impurity, which we are unaware of. In summary, the phosphate your client detected does not come from any storage buffer or anything we treated the protein with."

J'espère que ces informations vous seront utiles. Je reviendrai vers vous quand j'aurais déterminé si nou pouvons fournir une protéine His-taggée et contenant un atome de fer ou autre métal lourd.

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Question

The 17 kDa product would not seem to be quite large enough even for just the 3 IgG binding domains (˜195 AA), but anyway I’m interested in one of the other protein G catalog items.
The information you provided about quality systems doesn’t really answer my questions…

Can you provide Certificates of Analysis for last three batches (for example)?

What type of quality system is in place, such as controlled documents.

What is the approximate batch size produced? Is Abcam the manufacturer?

Most importantly, is there no function testing? How do you verify that the product is recombinant Protein G? Can you provide some function test data?
I would like to telephone tomorrow (Tuesday) to follow up. Can you recommend a time?

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Answer

I was forwarded your information by my colleague, and I'm happy to help out with your enquiries.

I'll be in touch with the manufacturing lab, and I can give you a call anytime this afternoon. Are you free around noon CDT?

Please let me know if there is another time that will work best for you. I look forward to speaking with you.

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