Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
Product nameRecombinant Protein G
See all Protein G proteins and peptides
Purity> 95 % SDS-PAGE.
>98% by SDS-PAGE and HPLC analyses. The albumin binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight26 kDa including tags
Amino acids190 to 384
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab49807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies.This protein contains only IgG binding domains.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cellular localizationCell Wall and Secreted
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab49807 has been referenced in 4 publications.
- Korodi M et al. Reusable on-plate immunoprecipitation method with covalently immobilized antibodies on a protein G covered microtiter plate. J Immunol Methods 483:112812 (2020). PubMed: 32569597
- Park S et al. Force-dependent trans-endocytosis by breast cancer cells depletes costimulatory receptor CD80 and attenuates T cell activation. Biosens Bioelectron 165:112389 (2020). PubMed: 32729511
- Li IT et al. Mapping cell surface adhesion by rotation tracking and adhesion footprinting. Sci Rep 7:44502 (2017). PubMed: 28290531
- Wang X et al. Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors. Sci Rep 6:21584 (2016). PubMed: 26875524