Recombinant Protein G (HRP) (ab7460)

Reviews (1)Q&A (1)References (5)
Western blot - Recombinant Protein G (HRP) (ab7460)
  • Dot Blot - Recombinant Protein G (HRP) (ab7460)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% Protein G purified
  • Suitable for: WB, Dot blot, ELISA, IHC-P

Description

  • Product name

    Recombinant Protein G (HRP)
    See all Protein G proteins and peptides

  • Purity

    > 98 % Protein G purified.
    Protein G Peroxidase was prepared from chromatographically pure Protein G.

  • Expression system

    Escherichia coli

  • Accession

    P19909

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Streptococcus

    • Sequence

      L DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLK

    • Predicted molecular weight

      63 kDa

    • Amino acids

      190 to 384

  • Conjugation

    HRP

Specifications

Our Abpromise guarantee covers the use of ab7460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

  • Form

    Lyophilized

  • Additional notes

    No reaction was observed against anti-Protein A.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA

  • Reconstitution
    Reconstitute product with 1ml of deionized water.

General Info

  • Alternative names

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all

  • Relevance

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

  • Cellular localization

    Cell Wall and Secreted

Images

  • Western blot - Recombinant Protein G (HRP) (ab7460)
    Western blot - Recombinant Protein G (HRP) (ab7460)Image courtesy of an anonymous Abreview.
    Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.

    See Abreview

  • Dot Blot - Recombinant Protein G (HRP) (ab7460)
    Dot Blot - Recombinant Protein G (HRP) (ab7460)
    Dot Blot analysis of ab7460 Protein G protein (HRP).

    Load: 200ng of ab7460 followed by a 3-fold serial dilution

    Antigens:
    Row 1: Human IgG
    Row 2: Rat IgG
    Row 3: Dog IgG

    Primary antibody: none
    Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature

    Block: Blocking buffer for 60 min at room temperature

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab7460? Please let us know so that we can cite the reference in this datasheet.

ab7460 has been referenced in 5 publications.

  • Simhadri VL  et al. Prevalence of Pre-existing Antibodies to CRISPR-Associated Nuclease Cas9 in the USA Population. Mol Ther Methods Clin Dev 10:105-112 (2018). PubMed: 30073181
  • Faburay B  et al. A glycosylated recombinant subunit candidate vaccine consisting of Ehrlichia ruminantium major antigenic protein1 induces specific humoral and Th1 type cell responses in sheep. PLoS One 12:e0185495 (2017). ELISA . PubMed: 28957443
  • Gocha T  et al. Identification and characterization of a novel Sso7d scaffold-based binder against Notch1. Sci Rep 7:12021 (2017). PubMed: 28931897
  • Hjerpe R  et al. UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance by the Proteasome. Cell 166:935-949 (2016). PubMed: 27477512
  • Zhang F  et al. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate. J Virol 80:10514-21 (2006). WB . PubMed: 17041222

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Other (IP+WB) Abreview for Protein G protein (HRP)

 Excellent
Abreviews
abreview image
Application
Other - Do not use

Review text: Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha on Protein G beads, and detection was with goat anti HP1-alpha, and Protein G HRP. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
Sample: Human Cell lysate - nuclear

Primary antibody (in addition to 'Protein G protein (HRP)')
Primary antibody: Abcam primary antibody: Anti-HP1 alpha antibody - ChIP Grade (ab77256)
Dilution: 1/2500

Secondary antibody
Name: Abcam antibody:Protein G protein (HRP)
Dilution: 1/10000

Abcam user community

Verified customer

Submitted Feb 24 2011

Question

band of interest is at about 27 kDda where light chain of endogenous IgG (from tissue samples) appears
send reference for using protein A-HRP or protein G-HRP to not detect the heavy and light chain of endogenous IgG in WB

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Answer

Thank you for contacting us.

Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


The links to the datasheets for protein A (HRP) and protein G (HRP) - which would both work well with a rabbit primary antibody are here:

https://www.abcam.com/Protein-A-protein-HRP-ab7456.html
https://www.abcam.com/Protein-G-protein-HRP-ab7460.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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