Description

  • Product name

    Recombinant rat BVR protein
    See all BVR proteins and peptides
  • Biological activity

    BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.

  • Purity

    > 95 % SDS-PAGE.
    Purified by Multi-Step Chromatography. > 95% pure as determined by SDS-PAGE and Western Blot analysis.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat

Associated products

Specifications

Our Abpromise guarantee covers the use of ab91622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    BVR Assay: Positive (> 180 units/mg) 1 unit of BVR will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.

    Protein previously labeled as Biliverdin Reductase.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.077% DTT, 0.79% Tris HCl, 0.0292% EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • BIEA_HUMAN
    • Biliverdin IX alpha reductase
    • Biliverdin reductase A
    • Biliverdin-IX alpha-reductase
    • BLVR
    • BLVR A
    • Blvra
    • BVR
    • BVR A
    • BVRA
    • Zinc metalloprotein
    see all
  • Function

    Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
  • Tissue specificity

    Liver.
  • Pathway

    Porphyrin-containing compound metabolism; protoheme degradation.
  • Involvement in disease

    Hyperbiliverdinemia
  • Sequence similarities

    Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight marker
    Lane 2: 0.5 µg ab91622
    Lane 3: 1.0 µg ab91622
    Lane 4: 2.0 µg ab91622
    Lane 5: 5.0 µg ab91622
  • All lanes : BVR Polyclonal Antibody

    Lane 1 : Molecular weight markers
    Lane 2 : ab91622 at 0.1 µg
  • BVR Activity: 1 µg of ab91622 was incubated with biliverdin in the presence of NADPH at pH 8.5, 37°C. Product formation was monitored with a spectrometer at 450 nm.

References

ab91622 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab91622.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up