Recombinant rat CaMKK protein (Tagged) (ab177255)
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Overview
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Product nameRecombinant rat CaMKK protein (Tagged)
See all CaMKK proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesRat
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SequenceMERSPAVCCQDPRAELVERVAAISVAHLEEAEEGPEPASNGVDPPPRARA ASVIPGSASRPTPVRPSLSARKFSLQERPAGSCLEAQVGPYSTGPASHMS PRAWRRPTIESHHVAISDTEDCVQLNQYKLQSEIGKGAYGVVRLAYNERE DRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAPQGGPAKQLLPLERVYQEI AILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFPEE QARLYLRDIILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQF EGNDAQLSSTAGTPAFMAPEAISDTGQSFSGKALDVWATGVTLYCFVYGK CPFIDEYILALHRKIKNEAVVFPEEPEVSEELKDLILKMLDKNPETRIGV SDIKLHPWVTKHGEEPLPSEEEHCSVVEVTEEEVKNSVKLIPSWTTVILV KSMLRKRSFGNPFEPQARREERSMSAPGNLLLKEGCGEGGKSPELPGVQE DEAAS
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Molecular weight88 kDa including tags
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Amino acids1 to 505
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Tagsproprietary tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab177255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
9 nmol/min/mg
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Applications
Western blot
SDS-PAGE
Functional Studies
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Purity> 85 % Densitometry.
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FormLiquid
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Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Ca2+/calmodulin-dependent protein kinase kinase alpha
- Calcium/calmodulin dependent protein kinase kinase
- Calcium/calmodulin dependent protein kinase kinase 1
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FunctionCalcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
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Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
DomainThe autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates. -
Post-translational
modificationsAppears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
Images
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Functional Studies - Recombinant rat CaMKK protein (Tagged) (ab177255)The specific activity of ab177255 was determined to be 9 nmol/min/mg.
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SDS-PAGE - Recombinant rat CaMKK protein (Tagged) (ab177255)SDS-PAGE analysis of ab177255.
Datasheets and documents
References
ab177255 has not yet been referenced specifically in any publications.
