Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: WB, SDS-PAGE, Functional Studies
Product nameRecombinant rat CaMKK protein (Tagged)
See all CaMKK proteins and peptides
Purity> 85 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMERSPAVCCQDPRAELVERVAAISVAHLEEAEEGPEPASNGVDPPPRARA ASVIPGSASRPTPVRPSLSARKFSLQERPAGSCLEAQVGPYSTGPASHMS PRAWRRPTIESHHVAISDTEDCVQLNQYKLQSEIGKGAYGVVRLAYNERE DRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAPQGGPAKQLLPLERVYQEI AILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFPEE QARLYLRDIILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQF EGNDAQLSSTAGTPAFMAPEAISDTGQSFSGKALDVWATGVTLYCFVYGK CPFIDEYILALHRKIKNEAVVFPEEPEVSEELKDLILKMLDKNPETRIGV SDIKLHPWVTKHGEEPLPSEEEHCSVVEVTEEEVKNSVKLIPSWTTVILV KSMLRKRSFGNPFEPQARREERSMSAPGNLLLKEGCGEGGKSPELPGVQE DEAAS
Predicted molecular weight88 kDa including tags
Amino acids1 to 505
Tagsproprietary tag N-Terminus
Our Abpromise guarantee covers the use of ab177255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.005% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Ca2+/calmodulin-dependent protein kinase kinase alpha
- Calcium/calmodulin dependent protein kinase kinase
- Calcium/calmodulin dependent protein kinase kinase 1
FunctionCalcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
modificationsAppears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab177255 has not yet been referenced specifically in any publications.