Recombinant rat CaMKK protein (Tagged) (ab177255)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: proprietary tag N-Terminus
- Suitable for: WB, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant rat CaMKK protein (Tagged)
See all CaMKK proteins and peptides -
Biological activity
9 nmol/min/mg
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Purity
> 85 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MERSPAVCCQDPRAELVERVAAISVAHLEEAEEGPEPASNGVDPPPRARA ASVIPGSASRPTPVRPSLSARKFSLQERPAGSCLEAQVGPYSTGPASHMS PRAWRRPTIESHHVAISDTEDCVQLNQYKLQSEIGKGAYGVVRLAYNERE DRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAPQGGPAKQLLPLERVYQEI AILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFPEE QARLYLRDIILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQF EGNDAQLSSTAGTPAFMAPEAISDTGQSFSGKALDVWATGVTLYCFVYGK CPFIDEYILALHRKIKNEAVVFPEEPEVSEELKDLILKMLDKNPETRIGV SDIKLHPWVTKHGEEPLPSEEEHCSVVEVTEEEVKNSVKLIPSWTTVILV KSMLRKRSFGNPFEPQARREERSMSAPGNLLLKEGCGEGGKSPELPGVQE DEAAS -
Predicted molecular weight
88 kDa including tags -
Amino acids
1 to 505 -
Tags
proprietary tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab177255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.005% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Ca2+/calmodulin-dependent protein kinase kinase alpha
- Calcium/calmodulin dependent protein kinase kinase
- Calcium/calmodulin dependent protein kinase kinase 1
see all -
Function
Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates. -
Post-translational
modificationsAppears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
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Functional Studies - Recombinant rat CaMKK protein (Tagged) (ab177255)
The specific activity of ab177255 was determined to be 9 nmol/min/mg.
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SDS-PAGE - Recombinant rat CaMKK protein (Tagged) (ab177255)SDS-PAGE analysis of ab177255.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab177255 has not yet been referenced specifically in any publications.
