Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Product nameRecombinant rat CCL4/MIP-1 beta protein
See all CCL4/MIP-1 beta proteins and peptides
Fully biologically active when compared to standard.
The biological activity determined by a chemotaxis bioassay using Human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
Purity> 95 % SDS-PAGE.
>95% by HPLC analysis.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight8 kDa
Amino acids24 to 92
Additional sequence informationSingle non-glycosylated polypeptide chain. This product is the mature full length protein from aa 24 to 92. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab201370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Macrophage Inflammatory Protein 1 beta, MIP1 beta
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 97% PBS, 3% Trehalose
Lyophilized from a 0.2µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionBriefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20°C to -70°C. Further dilutions should be made in appropriate buffered solutions.
- MIP 1 beta
- Secreted protein G 26
- ACT 2
FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201370 has not yet been referenced specifically in any publications.