Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Product nameRecombinant rat CX3CL1 protein (Active)
See all CX3CL1 proteins and peptides
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-10 ng/ml.
Purity> 95 % SDS-PAGE.
>95% as determined by SDS-PAGE and HPLC.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight9 kDa
Amino acids25 to 100
Our Abpromise guarantee covers the use of ab243779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
0.2 µm filtered
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at =-20 °C. Further dilutions should be made in appropriate buffered solutions.
- A 152E5.2
- ABCD 3
FunctionThe soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
Tissue specificitySmall intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the intercrine delta family.
modificationsA soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243779 has not yet been referenced specifically in any publications.