Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameRecombinant rat CXCL1/GRO alpha protein
See all CXCL1/GRO alpha proteins and peptides
Purity> 98 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per g (1EU/g).
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceAPVANELRCQCLQTVAGIHFKNIQSLKVMP PGPHCTQTEVIATLKNGREACLDPEAPMVQ KIVQKMLKGVPK
Predicted molecular weight11 kDa
Amino acids25 to 96
Our Abpromise guarantee covers the use of ab9773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The maximal chemotactic activity of rat CXCL1/GRO alpha on rat neutrophils was determined by its ability to chemoattract rat neutrophils using a concentration range of 10.0-100.0 ng/ml.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
FunctionHas chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
All lanes : Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/ml
Lane 1 :
Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.1 µg
Lane 2 :
Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.01 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9773 has not yet been referenced specifically in any publications.