Recombinant rat Cyclophilin F protein (Active) (ab219422)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant rat Cyclophilin F protein (Active)
See all Cyclophilin F proteins and peptides -
Biological activity
Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmol of suc-AAPF-PNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.
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Purity
> 90 % SDS-PAGE.
Purified by using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS -
Predicted molecular weight
21 kDa including tags -
Amino acids
29 to 206 -
Tags
His tag N-Terminus -
Additional sequence information
Mature full length form without signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab219422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
Functional Studies
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cyclophilin 3
- cyclophilin D
- Cyclophilin F
see all -
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219422 has not yet been referenced specifically in any publications.