Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant rat DNA Polymerase beta protein
See all DNA Polymerase beta proteins and peptides
Biological activityEnzyme activity: 90 unit/µl (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37°C in 60 min.)
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight38 kDa
Amino acids2 to 335
Our Abpromise guarantee covers the use of ab123225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2.28% Potassium chloride, 0.02% DTT, 0.79% Tris HCl, 0.003% EDTA, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- DNA directed DNA polymerase beta
- DNA pol beta
- DNA polymerase beta
FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
Cellular localizationNucleus, Cytoplasm
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123225 has been referenced in 1 publication.
- Date T et al. Expression of active rat DNA polymerase beta in Escherichia coli. Biochemistry 27:2983-90 (1988). PubMed: 3042024