Description

  • Product name

    Recombinant Rat G protein alpha S (mutated L227 + N295)
    See all GNAS proteins and peptides
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat

Specifications

Our Abpromise guarantee covers the use of ab90406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

  • Form

    Liquid
  • Additional notes


    GTP-binding possesses a highly conserved aspartate residue in the NKXD motif that is critical for high-affinity interaction with GTP. In almost all GTP-binding proteins so far, the D/N-mutation switches base-specifity from guanine to xanthine. Whereas the exchange of Asp295 to Asn295 leads to inactive mutants of Galpha-subunits, an additional Q/L-mutation in the catalytic site (Gln227 to Leu227) rescues protein function and induces xanthine nucleotide-specifity.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 0.11875% Magnesium chloride, 1.185% Tris HCl, 0.0292% EDTA

General Info

  • Alternative names

    • Adenylate cyclase stimulating G alpha protein
    • AHO
    • Alternative gene product encoded by XL exon
    • C20orf45
    • dJ309F20.1.1
    • dJ806M20.3.3
    • Extra large alphas protein
    • GNAS
    • GNAS complex locus
    • GNAS1
    • GPSA
    • Gs alpha subunit
    • GSA
    • GSP
    • Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1
    • Guanine nucleotide binding protein alpha stimulating activity polypeptide 1
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms short
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas
    • Guanine nucleotide regulatory protein
    • MGC33735
    • NESP
    • NESP55
    • Neuroendocrine secretory protein
    • PHP1A
    • PHP1B
    • PHP1C
    • POH
    • Protein ALEX
    • Protein GNAS
    • Protein NESP55
    • SCG6
    • Secretogranin VI
    • XLalphas
    • XLas
    see all
  • Relevance

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
  • Cellular localization

    Cell Membrane

References

ab90406 has not yet been referenced specifically in any publications.

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