Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Suitable for: Functional Studies
Product nameRecombinant Rat G protein alpha S (mutated L227 + N295)
See all GNAS proteins and peptides
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab90406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GTP-binding possesses a highly conserved aspartate residue in the NKXD motif that is critical for high-affinity interaction with GTP. In almost all GTP-binding proteins so far, the D/N-mutation switches base-specifity from guanine to xanthine. Whereas the exchange of Asp295 to Asn295 leads to inactive mutants of Galpha-subunits, an additional Q/L-mutation in the catalytic site (Gln227 to Leu227) rescues protein function and induces xanthine nucleotide-specifity.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.11875% Magnesium chloride, 1.185% Tris HCl, 0.0292% EDTA
- Adenylate cyclase stimulating G alpha protein
- Alternative gene product encoded by XL exon
RelevanceGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Cellular localizationCell Membrane
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab90406 has not yet been referenced specifically in any publications.