Description

  • Product name

    Recombinant rat GDNF protein
    See all GDNF proteins and peptides
  • Biological activity

    The activity of ab187229 is determined by the dose-dependent proliferation of C6 cells and is typically less than 0.1 μg/mL.

  • Purity

    >= 98 % SDS-PAGE.
    The purity of ab187229 is determined under reducing and non-reducing conditions.
  • Endotoxin level

    <=1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat
    • Sequence

      MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI
    • Predicted molecular weight

      30 kDa
    • Amino acids

      78 to 211
    • Additional sequence information

      Mature, non-glycosylated homodimer, containing two 135 amino acid chains.

Specifications

Our Abpromise guarantee covers the use of ab187229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    Constituent: 0.14% Sodium phosphate

    Sterile filtered.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. DO NOT VORTEX. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

General Info

  • Alternative names

    • Astrocyte derived trophic factor
    • Astrocyte derived trophic factor 1
    • Astrocyte-derived trophic factor
    • Atf
    • ATF 1
    • ATF 2
    • ATF1
    • ATF2
    • gdnf
    • GDNF_HUMAN
    • Glial cell derived neurotrophic factor
    • Glial Cell Line Derived Neurotrophic Factor
    • Glial cell line-derived neurotrophic factor
    • Glial derived neurotrophic factor
    • HFB1 GDNF
    • hGDNF
    • HSCR3
    see all
  • Function

    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • Tissue specificity

    In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • Involvement in disease

    Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • Sequence similarities

    Belongs to the TGF-beta family. GDNF subfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  •  SDS PAGE analysis of ab187229 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

References

ab187229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187229.
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