Recombinant rat Hsp70 protein (ab91602)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant rat Hsp70 protein
See all Hsp70 proteins and peptides -
Biological activity
ATPase Activity Assay: Positive -
Purity
> 90 % SDS-PAGE.
Purified by Multi-step chromatography. >90% pure as determined by SDS-PAGE and Western blot analyses. This protein does not contain DnaK as demonstrated by western blot analysis using anti-DnaK. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.0154% DTT, 0.79% Tris HCl, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Images
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SDS-PAGE - Recombinant rat Hsp70 protein (ab91602)SDS-PAGE Analysis of 0.5 μg ab91602
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Western blot - Recombinant rat Hsp70 protein (ab91602)All lanes : Anti-Hsp70 Monoclonal Antibody at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : ab91602 at 0.1 µg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91602 has not yet been referenced specifically in any publications.