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    recombinant-rat-hsp70-protein-ab91602.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Recombinant rat Hsp70 protein (ab91602)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant rat Hsp70 protein (ab91602)
  • Western blot - Recombinant rat Hsp70 protein (ab91602)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Suitable for: WB, Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant rat Hsp70 protein
    See all Hsp70 proteins and peptides
  • Biological activity

    ATPase Activity Assay: Positive
  • Purity

    > 90 % SDS-PAGE.
    Purified by Multi-step chromatography. >90% pure as determined by SDS-PAGE and Western blot analyses. This protein does not contain DnaK as demonstrated by western blot analysis using anti-DnaK.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat

Associated products

  • Related Products

    • Anti-Hsp70 antibody [5A5] (ab2787)
    • Anti-Hsp70 antibody [EP1007Y] (ab45133)
    • Anti-Hsp70 antibody [N27F3-4] (ab47454)
    • Anti-Hsp70 antibody [C92F3A-5] (ab47455)
    • Anti-Hsp70 antibody [3A3] (ab5439)
    • Anti-Hsp70 antibody [2A4] (ab5442)
    • Anti-Hsp70 antibody (ab79852)

Specifications

Our Abpromise guarantee covers the use of ab91602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.0154% DTT, 0.79% Tris HCl, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • DnaK type molecular chaperone HSP70 1
    • Epididymis secretory protein Li 103
    • FLJ54303
    • FLJ54370
    • FLJ54392
    • FLJ54408
    • FLJ75127
    • Heat shock 70 kDa protein 1
    • Heat shock 70 kDa protein 1/2
    • Heat shock 70 kDa protein 1A
    • Heat shock 70 kDa protein 1A/1B
    • Heat shock 70kDa protein 1B
    • Heat shock induced protein
    • HEL S 103
    • HSP70 1
    • HSP70 1A
    • HSP70 1B
    • HSP70 2
    • HSP70-1
    • HSP70-1/HSP70-2
    • HSP70-1A
    • HSP70-2
    • HSP70.1
    • HSP70.1/HSP70.2
    • HSP71_HUMAN
    • HSP72
    • HSPA1
    • HSPA1A
    • HSPA1B
    see all
  • Relevance

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Images

  • SDS-PAGE - Recombinant rat Hsp70 protein (ab91602)
    SDS-PAGE - Recombinant rat Hsp70 protein (ab91602)
    SDS-PAGE Analysis of 0.5 μg ab91602
  • Western blot - Recombinant rat Hsp70 protein (ab91602)
    Western blot - Recombinant rat Hsp70 protein (ab91602)
    All lanes : Anti-Hsp70 Monoclonal Antibody at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : ab91602 at 0.1 µg

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab91602? Please let us know so that we can cite the reference in this datasheet.

ab91602 has not yet been referenced specifically in any publications.

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