Recombinant Rat IGF1 protein (ab52006)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Rat IGF1 protein
See all IGF1 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Greater than 95% by SDS-PAGE and HPLC analyses. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat
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Associated products
Specifications
Our Abpromise guarantee covers the use of ab52006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
This solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. The lyophilized IGF-I is best-stored desiccated below 0°C. Reconstituted IGF-I should be stored at working aliquots at -20°C. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Endotoxin level is <0.1 ng per µg of IGF-I. -
ReconstitutionReconstitute in water to a concentration of 0.1-1.0 mg/ml.
General Info
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Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (2)
ab52006 has been referenced in 2 publications.
- Chen H et al. Prolyl hydroxylase 2 silencing enhances the paracrine effects of mesenchymal stem cells on necrotizing enterocolitis in an NF-?B-dependent mechanism. Cell Death Dis 11:188 (2020). PubMed: 32179740
- Yang X et al. IGF-1 protects retinal ganglion cells from hypoxia-induced apoptosis by activating the Erk-1/2 and Akt pathways. Mol Vis 19:1901-12 (2013). PubMed: 24049436
