Recombinant rat IL-10 protein (Active) (ab9970)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/g
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant rat IL-10 protein (Active)
See all IL-10 proteins and peptides -
Biological activity
Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation. Dose is lot dependent.
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Purity
> 98 % SDS-PAGE.
Sterile filtered. Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g). Lot dependent. -
Endotoxin level
< 1.000 Eu/g -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
Interleukin-10, originally known as Cytokine Synthesis Inhibitory factor (CSIF), is an 18.7 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI: SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN -
Amino acids
19 to 178
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab9970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
The ED50, determined by the dose-dependent inhibition of antigen-specific T cell proliferation, is < 10 ng/ml -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.328% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
General Info
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab9970 has not yet been referenced specifically in any publications.