Recombinant rat IL-15 protein (ab49905)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant rat IL-15 protein
See all IL-15 proteins and peptides -
Biological activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of murine CTLL-2 cells is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
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Purity
> 95 % SDS-PAGE.
Greater than 96% by SDS-PAGE and HPLC analyses. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MNWIDVRYDL EKIESLIQFI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VIESGCKECE ELEERNFTEF LQSFIHIVQM FINTS
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Specifications
Our Abpromise guarantee covers the use of ab49905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 50 mM Tris, pH 8.5 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
General Info
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Alternative names
- IL 15
- IL-15
- IL15
see all -
Function
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. -
Tissue specificity
Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab49905 has not yet been referenced specifically in any publications.