Recombinant rat IL-1RA protein (ab200257)
Key features and details
- Expression system: Escherichia coli
- Purity: > 96% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Description
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Product name
Recombinant rat IL-1RA protein
See all IL-1RA proteins and peptides -
Biological activity
The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg in the presence of 50 pg/ml rat IL-1RA.
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Purity
> 96 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ -
Predicted molecular weight
17 kDa -
Amino acids
27 to 178 -
Additional sequence information
This product is for the mature full length protein from aa 27 to 178. The signal peptide is not included.
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Specifications
Our Abpromise guarantee covers the use of ab200257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
HPLC
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS
Lyophilized from a 0.2 µm filtered concentrated solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL
General Info
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Alternative names
- DIRA
- F630041P17Rik
- ICIL 1RA
see all -
Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
Tissue specificity
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
Involvement in disease
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
Cellular localization
Cytoplasm and Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab200257 has not yet been referenced specifically in any publications.