Bioactive grade

Recombinant rat IL-5 protein (Active) (ab200248)

Description

  • Product name

    Recombinant rat IL-5 protein (Active)
    See all IL-5 proteins and peptides
  • Biological activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

  • Purity

    > 98 % SDS-PAGE.
    Purity > 98 % by SDS-PAGE and HPLC analyses.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat
    • Sequence

      MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV
    • Predicted molecular weight

      26 kDa
    • Amino acids

      20 to 132
    • Additional sequence information

      A disulfide-linked homodimeric protein containing two 113 amino acids. This product is for the mature full length protein. The signal peptide is not included.

Specifications

Our Abpromise guarantee covers the use of ab200248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.

    pH: 7.40
    Constituent: 100% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at = -20 °C. Further dilutions should be made in appropriate buffered solutions.

General Info

  • Alternative names

    • B-cell differentiation factor I
    • Colony stimulating factor
    • EDF
    • Eosinophil differentiation factor
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor, eosinophil)
    • Interleukin-5
    • T Cell Replacing Factor
    • T-cell replacing factor
    • TRF
    see all
  • Function

    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • Sequence similarities

    Belongs to the IL-5 family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab200248 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab200248.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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