Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 58% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant rat ITK/EMT protein
See all ITK/EMT proteins and peptides
Specific Activity: 1.0 pmol/min/μg
Purity>= 58 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequencePVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQE GAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSD YLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQV IKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFG VLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASPHVYQVMNHCW KEKPEDRPPFSRLLSQLAE
Predicted molecular weight57 kDa including tags
Amino acids352 to 620
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab196125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as ITK.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.4% Tris HCl, 0.58% Sodium chloride, 0.05% Tween, 0.05% DTT, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Homolog of mouse T cell itk/tsk
- IL 2 inducible T cell kinase
FunctionPlays a role in T-cell proliferation and differentiation.
Tissue specificityT-cell lines and natural killer cell lines.
Involvement in diseaseDefects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Cellular localizationCell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
- Information by UniProt
10% SDS-PAGE analysis of ab196125 with Coomassie staining.
Lane 1: 20 µg Active rat ITK/EMT protein fragment (ab196125)
Lane 2: Protein marker
Enzyme reaction was conducted at 30°C for 1 hour with 2 μM Tyr peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour incubation, diluted development reagent was added. Percentage phosphorylation was calculated based on the emission ratio of 445/520 nm after excitation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196125 has not yet been referenced specifically in any publications.