Key features and details
- Expression system: Escherichia coli
- Purity: = 95% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant rat M-CSF protein (Animal Free)
See all M-CSF proteins and peptides
NFS-60 cell proliferation: ED50 ≤10 ng/ml; ≥ 1.0 x 105 units/mg.
Purity= 95 % SDS-PAGE.
Purity is typically 95% as determined by reducing and non-reducing SDS-PAGE.
Endotoxin level< 0.050 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMEVSEHCSHMIGNGHLQILQQLIDSQMETACLIEYKFVDQEQLDDPVCYL KKAFVLVQVIIEETMRFKDNTPNANATERLQELSMKLNSCFIKDYKEQNE ACVQTYKESPLRLLEKIKNFFNETKNFLEKDWNIFSKNCNDSLAKCSSRD VVTKP
Predicted molecular weight36 kDa
Amino acids33 to 186
Additional sequence informationNon-glycosylated homodimer.
Our Abpromise guarantee covers the use of ab207964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile water to 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. Dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab207964 has not yet been referenced specifically in any publications.