Description

  • Product name

    Recombinant rat PAI1 protein (Biotin)
    See all PAI1 proteins and peptides
  • Biological activity

    ab170081 typically contains 50 percent active PAI1.
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat
    • Sequence

      SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTT AGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQ RDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISD LLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVP MMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAI TNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFS STQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPT EMVLDRSFLFVVRHNPTETILFMGQLMEP
    • Predicted molecular weight

      43 kDa
    • Amino acids

      24 to 402
  • Conjugation

    Biotin

Specifications

Our Abpromise guarantee covers the use of ab170081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 5.00
    Constituents: 0.41% Sodium acetate, 0.03% EDTA, 2.34% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • Nexin plasminogen activator inhibitor type 1
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serine proteinase inhibitor clade E member 1
    • serpin
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all
  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab170081.

    Lane 1: 3µg ab170081 Reduced
    Lane 2: Rat PAI1 (3µg) + Urokinase (15µg) Reduced
    Lane 3: Molecular weight markers

  • Avidin-Alkaline Phosphatase Western Blot analysis of ab170081.

    Lane 1: 0.1µg ab170081 Reduced
    Lane 2: Rat PAI1 (3µg) + Urokinase (2µg) Reduced

References

ab170081 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170081.
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