Recombinant rat PCSK9 protein (His tag) (ab201424)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant rat PCSK9 protein (His tag)
See all PCSK9 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant human LDL Receptor protein (ab220582) can bind Recombinant Rat PCSK9 protein (ab201424) with a linear range of 8-63 ng/mL.
Measured by its binding ability in a BLI assay. Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant rat PCSK9 protein (ab201424) with an affinity constant of 1.61 nM.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
QDEDGDYEELMLALPSQEDSLVDEASHVATATFRRCSKEAWRLPGTYVVV LMEETQRLQV EQTAHRLQTWAARRGYVIKVLHVFYDLFPGFLVKMSSD LLGLALKLPHVEYIEEDSLVFAQSIPWNLERIIPAWQQTEEDSSPDGSSQ VEVYLLDTSIQSGHREIEGRVTITDFNSVPEED GTRFHRQASKCDSHG THLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIR KSQ LIQPSGPLVVLLPLAGGYSRILNTACQRLARTGVVLVAAAGNFRDDACLY SPASAPE VITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASS DCSTCYMSQSGTSQAAAHV AGIVAMMLNRDPALTLAELRQRLILFSTK DVINMAWFPEDQRVLTPNRVATLPPSTQETG GQLLCRTVWSAHSGPTR TATATARCAPEEELLSCSSFSRSGRRRGDRIEAIGGQQVCKAL NAFGG EGVYAVARCCLLPRVNCSIHNTPAARAGPQTPVHCHQKDHVLTGCSFHWE VENLR AQQQPLLRSRHQPGQCVGHQEASVHASCCHAPGLECKIKEHGI AGPAEQVTVACEAGWTL TGCNVLPGASLPLGAYSVDNVCVARIRDAGR ADRTSEEATVAAAICCRSRPSAKASWVHQ -
Predicted molecular weight
73 kDa including tags -
Amino acids
31 to 691 -
Tags
His tag C-Terminus -
Additional sequence information
Mature form including propeptide. The signal peptide is not included. (NP_954862).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab201424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
General Info
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Alternative names
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
see all -
Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
Post-translational
modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
Cellular localization
Secreted. - Information by UniProt
Images
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Immobilized Recombinant human LDL Receptor protein (ab220582) can bind Recombinant Rat PCSK9 protein (ab201424) with a linear range of 8-63 ng/mL.
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SDS-PAGE analysis of reduced ab201424 stained overnight with Coomassie Blue. The protein migrates as 16 kDa and 60-66 kDa under reducing condition due to glycosylation.
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Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant rat PCSK9 protein (ab201424) with an affinity constant of 1.61 nM as determined in BLI assay.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab201424 has not yet been referenced specifically in any publications.