Recombinant rat PDE1B protein (ab198476)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 35% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant rat PDE1B protein
See all PDE1B proteins and peptides -
Biological activity
Specific Activity: ≥ 6800 pmol/min/µg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198476 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
>= 35 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEV NIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWL ASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTAVGPTYS TAVHNCLKNLDVWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPT VFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEI EVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVF RMMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQ QLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAE LGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLTDDD SKSKSQPSFQWRQPSLDVDVGDPNPDVVSFRSTWTKYIQENKQKWKERAA SGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD -
Predicted molecular weight
89 kDa including tags -
Amino acids
1 to 535 -
Tags
His tag N-Terminus , GST tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 20% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic nucleotide phosphodiesterase 1B
- 63 kDa Cam PDE
- 63 kDa Cam-PDE
see all -
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab198476 has not yet been referenced specifically in any publications.