Recombinant rat PDE1B protein (ab198476)

Submit a review Submit a question
Functional Studies - Recombinant rat PDE1B protein (ab198476)
  • SDS-PAGE - Recombinant rat PDE1B protein (ab198476)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 35% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus, GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant rat PDE1B protein
    See all PDE1B proteins and peptides

  • Biological activity

    Specific Activity: ≥ 6800 pmol/min/µg

    Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198476 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

  • Purity

    >= 35 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q01066

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rat

    • Sequence

      MELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEV NIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWL ASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTAVGPTYS TAVHNCLKNLDVWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPT VFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEI EVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVF RMMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQ QLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAE LGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLTDDD SKSKSQPSFQWRQPSLDVDVGDPNPDVVSFRSTWTKYIQENKQKWKERAA SGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD

    • Predicted molecular weight

      89 kDa including tags

    • Amino acids

      1 to 535

    • Tags

      His tag N-Terminus , GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab198476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.36% Imidazole
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 5''-cyclic nucleotide phosphodiesterase 1B
    • 63 kDa Cam PDE
    • 63 kDa Cam-PDE
    • Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B
    • Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase
    • Calcium/calmodulin-dependent 3''
    • Calmodulin stimulated phosphodiesterase PDE1B1
    • Cam PDE 1B
    • Cam PDE1B
    • Cam-PDE 1B
    • PDE 1B
    • PDE1B
    • PDE1B_HUMAN
    • PDE1B1
    • PDES 1B
    • PDES1B
    • Phosphodiesterase 1B
    • Phosphodiesterase 1B calmodulin dependent
    • Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B
    see all

  • Function

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant rat PDE1B protein (ab198476)
    Functional Studies - Recombinant rat PDE1B protein (ab198476)

    Example of specific activity of ab198476

  • SDS-PAGE - Recombinant rat PDE1B protein (ab198476)
    SDS-PAGE - Recombinant rat PDE1B protein (ab198476)

    10% SDS-PAGE analysis of ab198476 with Coomassie staining.

    Lane 1: 2.7 μg ab198476
    Lane 2: Protein marker

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab198476? Please let us know so that we can cite the reference in this datasheet.

ab198476 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab198476.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com