Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 35% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant rat PDE1B protein
See all PDE1B proteins and peptides
Specific Activity: ≥ 6800 pmol/min/µg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198476 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Purity>= 35 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEV NIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWL ASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTAVGPTYS TAVHNCLKNLDVWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPT VFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEI EVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVF RMMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQ QLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAE LGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLTDDD SKSKSQPSFQWRQPSLDVDVGDPNPDVVSFRSTWTKYIQENKQKWKERAA SGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD
Predicted molecular weight89 kDa including tags
Amino acids1 to 535
TagsHis tag N-Terminus , GST tag N-Terminus
Our Abpromise guarantee covers the use of ab198476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic nucleotide phosphodiesterase 1B
- 63 kDa Cam PDE
- 63 kDa Cam-PDE
FunctionCyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198476 has not yet been referenced specifically in any publications.