Recombinant rat PDE7B protein (ab198460)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant rat PDE7B protein
See all PDE7B proteins and peptides -
Biological activity
Specific Activity: ≥324 pmol/min/µg.
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198460 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
30 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MLSKVGMWDFDIFLFDRLTNGNSLVTLLCHLFNSHGLIHHFKLDMVTLHR FLVMVQEDYHGHNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGL LAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRES RLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLTRLKAHLHNKDLRLEN IQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGDLEQKFEL EISPLCNQQKDSIPSIQIGFMTYIVEPLFREWARFTGNSTLSESMLNHLA HNKAQWKSLLSNQHRRRGSGQDPAGTAPETLEQTEGATP -
Predicted molecular weight
65 kDa including tags -
Amino acids
108 to 446 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_080894.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.55% Glutathione, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- bA472E5.1
- cAMP specific 3 5 cyclic phosphodiesterase 7B
- cAMP specific 3'5' cyclic phosphodiesterase 7B
see all -
Relevance
PDE7B is a cAMP specific phosphodiesterase. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE7B has three splice variants (PDE7B1 = 446 amino acid; PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences. PDE7B is expressed in various tissues including skeletal muscle, heart, lung and testis. PDE7B may be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain. -
Cellular localization
cytosol
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198460 has not yet been referenced specifically in any publications.