Recombinant rat PTP1B protein (ab42573)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Inhibition Assay

Description

  • Product name

    Recombinant rat PTP1B protein
    See all PTP1B proteins and peptides

  • Biological activity

    The specific activity of rat PTP1B was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 5 min incubation at 30C, 2 ug/ml enzyme.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rat

    • Amino acids

      1 to 321

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab42573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Inhibition Assay

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • PTP1B
    • Non receptor tyrosine phosphatase 1
    • Protein phosphotyrosylphosphatase 1B
    • Protein tyrosine phosphatase 1B
    • Protein tyrosine phosphatase non receptor type 1
    • Protein tyrosine phosphatase placental
    • Protein-tyrosine phosphatase 1B
    • PTN1_HUMAN
    • PTP 1B
    • PTP-1B
    • PTPN 1
    • PTPN1
    • Tyrosine protein phosphatase non receptor type 1
    • Tyrosine-protein phosphatase non-receptor type 1
    see all

  • Function

    May play an important role in CKII- and p60c-src-induced signal transduction cascades.

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.

  • Post-translational
    modifications

    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab42573? Please let us know so that we can cite the reference in this datasheet.

ab42573 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

20 ug protein
what is the volume?

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Answer

Thank you for contacting us.

The concentration of the protein is 3 mg/ml. Thus, the volume is 6.67 ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

will test ab42573 in WB

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Answer

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab42573 in WB. This code will give you: 1 freePROTEIN before the expiration date. To redeem this offer, please submit an Abreview for WBand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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