Description

  • Product name

    Recombinant rat Thyroid Peroxidase/TPO protein
    See all Thyroid Peroxidase/TPO proteins and peptides
  • Biological activity

    Determined by its ability to stimulate the proliferation of Human MO7e cells. The expected ED50 is = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
  • Purity

    > 98 % SDS-PAGE.
    Purity: > 98% by SDS-PAGE and HPLC analysis.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Rat
    • Sequence

      SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
    • Predicted molecular weight

      19 kDa

Specifications

Our Abpromise guarantee covers the use of ab129140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilised
  • Additional notes

    Endotoxin level: < 0.1 ng/µg of Thyroid Peroxidase/TPO.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstituted ab129140 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

General Info

  • Alternative names

    • MSA
    • PERT_HUMAN
    • TDH2A
    • Thyroid microsomal antigen
    • Thyroid peroxidase
    • Thyroperoxidase
    • TPO
    • TPX
    see all
  • Function

    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • Pathway

    Hormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in disease

    Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications

    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localization

    Membrane and Cell surface.
  • Information by UniProt

References

ab129140 has not yet been referenced specifically in any publications.

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